RAB0223

Human IFN γ ELISA Kit

Name: Human IFN γ ELISA Kit
Brand: SIGMA
Price: 608 USD

for cell and tissue lysates

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1 KIT

$608.00

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About This Item

UNSPSC Code:

41116158

NACRES:

NA.77

Key Documents

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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type tissue lysate
sample type cell lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 20.6-15000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IFNG(3458)

Related Categories

Enzyme-Linked Immunosorbent Assay (ELISA) Kits

Immunoassay Platform Solutions

Protein Biology

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This Item	RAB0222	RAB0225	RAB0224
Sigma-Aldrich RAB0223 Human IFN γ ELISA Kit	Sigma-Aldrich RAB0222 Human IFN γ ELISA Kit	Sigma-Aldrich RAB0225 Mouse IFN-γ ELISA Kit	Sigma-Aldrich RAB0224 Mouse IFN-γ ELISA Kit
species reactivity human	species reactivity human	species reactivity mouse	species reactivity mouse
Gene Information human ... IFNG(3458)	Gene Information human ... IFNG(3458)	Gene Information mouse ... Ifng(15978)	Gene Information mouse ... Ifng(15978)
assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 15 pg/mL standard curve range: 20.6-15000 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 15 pg/mL standard curve range: 20.6-15000 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 10 pg/mL standard curve range: 2.74-2000 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 5 pg/mL standard curve range: 2.74-2000 pg/mL
input sample type tissue lysate sample type cell lysate	input sample type plasma sample type urine sample type serum sample type cell culture supernatant(s)	input sample type cell lysate sample type tissue lysate	input sample type cell culture supernatant(s) sample type serum sample type plasma
technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

General description

The Human IFN-γ ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IFN-γ cell lysate and tissue lysate.

Immunogen

Recombinant Human IFN-γ

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human IFNγ ELISA Kit has been used in ELISA assay.[1]

Biochem/physiol Actions

IFNG (interferon-γ) is a type-2 interferon, which is produced by Th (T helper cells)-1 lymphocytes, CD8 cytotoxic lymphocytes, natural killer (NK) cells, and natural killer T (NKT) cells. It plays an important role in innate and adaptive immune responses, and helps in defense against bacterial infections, dimorphic yeast and some viral infections. High expression of IFNG is common in many autoimmune diseases, such as systemic lupus erythematosis, Sjögren syndrome, inflammatory bowel disease and multiple sclerosis.[2]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.

Description

SDS

RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)SDS

RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

RABLYSIS12X Cell Lysis Buffer (Item J)SDS

RABSTOP3ELISA Stop Solution (Item I)SDS

RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

RABWASH420X Wash Buffer (Item B)SDS

pictograms

GHS05

signalword

Warning

hcodes

H290

pcodes

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class

8B - Non-combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

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Early or late IL-10 blockade enhances Th1 and Th17 effector responses and promotes fungal clearance in mice with cryptococcal lung infection.

Benjamin J Murdock et al.

Journal of immunology (Baltimore, Md. : 1950), 193(8), 4107-4116 (2014-09-17)

The potent immunoregulatory properties of IL-10 can counteract protective immune responses and, thereby, promote persistent infections, as evidenced by studies of cryptococcal lung infection in IL-10-deficient mice. To further investigate how IL-10 impairs fungal clearance, the current study used an

Sustained inhibition of HIV-1 replication by conditional expression of the E. coli-derived endoribonuclease MazF in CD4+ T cells.

Mika Okamoto et al.

Human gene therapy methods, 24(2), 94-103 (2013-02-28)

Gene therapy using a Tat-dependent expression system of MazF, an ACA nucleotide sequence-specific endoribonuclease derived from Escherichia coli, in a retroviral vector appears to be an alternative approach to the treatment of human immunodeficiency virus type 1 (HIV-1) infection. MazF

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