RAB0229

Sigma-Aldrich

Mouse IGF-I ELISA Kit

for serum, plasma and cell culture supernatant

NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

application(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 4 pg/mL
standard curve range: 2.74-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Igf1(16000)

General description

The Mouse IGF-I ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IGF-I in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Mouse IGF-I

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse IGF-I ELISA Kit has been used to measure the concentration of insulin-like growth factor(IGF) secreted by mesenchymal stem cells(MSCs). It has also been used to measure the level of IGF1 in blood samples of mice.
For research use only. Not for use in diagnostic procedures.
Mouse IGF-I ELISA kit has been used to measure plasma IGF-I (Insulin-like growth factor-I) level from Igf1r L/L and BAT-specific Igf1r-knockout (BATIGFIRKO) mice.

Biochem/physiol Actions

IGF-I (Insulin-like growth factor-I) is required for fetal growth and development. It is also involved in the growth and repair of heart tissues. IGF-I has been linked to neuroplasticity and hippocampal neurogenesis. It is important in the recovery from ischaemic injury. IGF-I also facilitates the differentiation of adipocytes and other cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

hazcat

Met. Corr. 1

storage_class_code

8B - Non-combustible, corrosive hazardous materials

Certificate of Analysis

Certificate of Origin

Flavia R Siqueira et al.
Physiology & behavior, 154, 68-75 (2015-11-26)
A low-salt (LS) diet during pregnancy has been linked to insulin resistance in adult offspring, at least in the experimental setting. However, it remains unclear if this effect is due to salt restriction during early or late pregnancy. To better...
María Llorens-Martín et al.
The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry, 15(2), 134-148 (2009-03-25)
This review addresses the role of serum insulin-like growth factor 1 (IGF1) as one mechanism of adult neural plasticity, specifically, its regulation of hippocampal neurogenesis among other plasticity-related processes. It is suggested that IGF has been reused advantageously both for...
B A Taylor et al.
Cytogenetics and cell genetics, 56(1), 57-58 (1991-01-01)
The peptide hormone, insulin-like growth factor I (IGF-I), is a major determinant of growth in mammals, and also plays a role in differentiation of adipocytes and other cells (Van Wyk, 1984). Although IGF-I is synthesized in many cell types, the...
He He et al.
Inflammation, 38(6), 2178-2184 (2015-06-25)
Sepsis-induced myocardial injury (SIMI) is caused by various mechanisms. The aim of this study was to investigate the effects of salidroside (Sal) on SIMI and its mechanisms in rats. The sepsis model was established by intraperitoneal injection of lipopolysaccharide (LPS)...
Pablo Roman-Garcia et al.
The Journal of clinical investigation, 124(7), 2988-3002 (2014-06-10)
Both maternal and offspring-derived factors contribute to lifelong growth and bone mass accrual, although the specific role of maternal deficiencies in the growth and bone mass of offspring is poorly understood. In the present study, we have shown that vitamin...

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