MilliporeSigma
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RAB0229

Sigma-Aldrich

Mouse IGF-I ELISA Kit

for serum, plasma and cell culture supernatant

Synonym(s):
Insulin-like growth factor I, Mechano growth factor, Somatomedin-C
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 4 pg/mL
standard curve range: 2.74-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Igf1(16000)

General description

The Mouse IGF-I ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IGF-I in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Mouse IGF-I

Application

For research use only. Not for use in diagnostic procedures.
Mouse IGF-I ELISA kit has been used to measure plasma IGF-I (Insulin-like growth factor-I) level from Igf1r L/L and BAT-specific Igf1r-knockout (BATIGFIRKO) mice.
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse IGF-I ELISA Kit has been used to measure the concentration of insulin-like growth factor(IGF) secreted by mesenchymal stem cells(MSCs). It has also been used to measure the level of IGF1 in blood samples of mice.

Biochem/physiol Actions

IGF-I (Insulin-like growth factor-I) is required for fetal growth and development. It is also involved in the growth and repair of heart tissues. IGF-I has been linked to neuroplasticity and hippocampal neurogenesis. It is important in the recovery from ischaemic injury. IGF-I also facilitates the differentiation of adipocytes and other cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

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