RAB0257

Mouse IL-13 ELISA Kit

for serum, plasma and cell culture supernatant

• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: mouse ... Il13(16163)
• Input: sample type serum; sample type cell culture supernatant(s); sample type plasma
• Species reactivity: mouse
• Storage temp.: −20°C
• Shipped in: wet ice

Properties

• species reactivity: mouse
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type serum; sample type cell culture supernatant(s); sample type plasma
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 6 pg/mL; standard curve range: 8023-2000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Il13(16163)

Description

General description

The Mouse IL-13 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-13 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Mouse IL-13

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Mouse IL-13 ELISA Kit has been used to measure the concentration of interleukin (IL)-13 in serological sample[1]and bronchoalveolar lavage fluids (BALFs).[2]

Biochem/physiol Actions

Interleukin 13 (IL13) is up-regulated during parasitic infections and participates in host′s protective immunity against Schistosoma japonicum. This protein promotes eosinophil activation resulting in elevated specific IgE against adult and larval antigens. During acute infection it promotes egg expulsion through increased intestinal contractility. It acts as a liver protective agent where it promotes the formation of liver granulomas in a CD4⁺ T cell-mediated manner, which results in liver fibrosis.[3] In airway disorders, such as asthma, chronic obstructive pulmonary disease (COPD), and cystic fibrosis lung disease, IL13 promotes the formation of airway goblet cells, resulting in excess mucus secretion, which in turn, is regulated by IL13-activated autophagy.[4]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABELADA ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
• RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 8A - Combustible corrosive hazardous materials