MilliporeSigma
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RAB0273

Sigma-Aldrich

Human IL-1 β ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):
Il-1 beta, Interleukin-1 beta

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type urine
sample type serum
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.3 pg/mL
standard curve range: 0.48-100 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL1B(3553)

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RAB0283RAB0279RAB0270
species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type urine
sample type serum
sample type plasma
sample type cell culture supernatant(s)

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma
sample type urine

input

sample type plasma
sample type urine
sample type serum
sample type cell culture supernatant(s)

input

sample type cell lysate
sample type tissue lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.3 pg/mL
standard curve range: 0.48-100 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 100 pg/mL
standard curve range: 0.124-30 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 8.19-2000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.5 pg/mL
standard curve range: 0.412-300 pg/mL

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The Human IL-1 β ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-1 β in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human IL-1β

Application

For research use only. Not for use in diagnostic procedures.
Human IL-1 β ELISA kit has been used to measure the concentration of interleukin 1β (IL-1 β) in cell culture supernatants.

Biochem/physiol Actions

Interleukin 1β (IL-1 β) is a proinflammatory cytokine, which is implicated in induction of various acute and chronic inflammation. Hence, IL1B can be considered as a potential therapeutic target for disease associated with fibrosis and tissue remodeling. IL-1β protein increases the production of endogenous 92kDa gelatinase (matrix metalloproteinases 9 (MMP-9)) and tissue inhibitors of MMP (TIMP -1). Elevated concentration of IL-1β in synovial fluid contributes to the pathogenesis of synovitis and degenerative changes of the cartilaginous tissue and bone of the temporomandibular joint. IL-1β brings neutrophil and macrophage to the site of infection. Mutations in IL-1β gene are linked with renal manifestations and renal sequelae in Henoch-Schönlein purpura (HSP).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3


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