RAB0306

Human IL-6 ELISA Kit

for serum, plasma, cell culture supernatant and urine

• Synonym(s): B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IL-6, Interferon beta-2, Interleukin-6
• NACRES: NA.32

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable; capture ELISA: suitable
• input: sample type urine; sample type cell culture supernatant(s); sample type plasma; sample type serum
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 3 pg/mL; standard curve range: 1.37-1000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... IL6(3569)

Description

General description

The Human IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-6 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human I-L6

Application

Human IL-6 ELISA kit has been used for determining the levels of IL-6 in biological samples.
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Packaging

1 kit

Biochem/physiol Actions

IL-6 (interleukin 6) is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 is associated with various autoimmune and inflammatory diseases. High levels of IL-6 are present in the synovial fluid from rheumatoid arthritis patients. It is also linked with chronic inflammatory proliferative disease and B cell malignancy, Castleman′s disease, and plasmacytoma/multiple myeloma.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Disclaimer

For research use only. Not for use in diagnostic procedures.

Kit Components Also Available Separately

• RABELADA ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
• RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Statements: H290
• Precautionary Statements: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class Code: 8A - Combustible, corrosive hazardous materials
• WGK: WGK 3