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RAB0308

Sigma-Aldrich

Mouse IL-6 ELISA Kit

for serum, plasma and cell culture supernatant

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Synonym(s):
B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IL-6, Interferon beta-2

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 0.82-600 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Il6(24498)

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Mouse IL-6 ELISA Kit for serum, plasma and cell culture supernatant

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Mouse IL-6 ELISA Kit

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Mouse IL-2 ELISA Kit

Mouse IL-4 ELISA Kit for serum, plasma and cell culture supernatant

RAB0299

Mouse IL-4 ELISA Kit

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

input

sample type tissue lysate
sample type cell lysate

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 0.82-600 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 0.82-600 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.625-40 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.6 pg/mL
standard curve range: 0.82-200 pg/mL

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

General description

The Mouse IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-6 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Mouse IL-6

Application

For research use only. Not for use in diagnostic procedures.
Mouse IL-6 ELISA kit has been used for determining the levels of IL-6 in biological samples.

Biochem/physiol Actions

IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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Effect of HI-6 on cytokines production after immunity stimulation by keyhole limpet hemocyanin in a mouse model.
Pohanka M
Neuro Endocrinology Letters, 35, 155-157 (2014)
The biology of interleukin-6.
Kishimoto T
Blood, 74(1), 1-10 (1989)
Minjie Ju et al.
Molecular medicine reports, 20(4), 3347-3354 (2019-08-23)
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Rubbel Singla et al.
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