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RAB0342

Sigma-Aldrich

Cell-Based ELISA for detecting phospho-P38 MAPK (pThr180 / pTyr182) in cultured cell lines

96 assays

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NACRES:
NA.32

usage

96 assays

species reactivity

human, rat, mouse

packaging

kit of 96 assay(s) (1 x 96 well plate)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cultured cells (96 assays)

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MAPK14(1432)

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RAB0344RAB0345RAB0350
species reactivity

human, rat, mouse

species reactivity

human, mouse

species reactivity

human, mouse

species reactivity

rat, human, mouse

packaging

kit of 96 assay(s) (1 x 96 well plate)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

-

packaging

-

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cultured cells (96 assays)

input

sample type tissue lysate
sample type cell lysate

input

sample type cell lysate
sample type tissue lysate

input

-

detection method

colorimetric

detection method

colorimetric

detection method

-

detection method

-

General description

The Cell-Based p38 MAPK (pThr180 / pTyr182) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of p38 MAPK (pThr180 / pTyr182) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.

Immunogen

p38 MAPK (pThr180 / pTyr182) synthetic phosphopeptide

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABBLOCK5x Blocking Solution (Item F)SDS

  • RABFIX1Fixing Solution (Item D)SDS

  • RABHRP1HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1)SDS

  • RABHRP2HRP-conjugated Anti-Mouse IgG Concentrate (Item I2)SDS

  • RABPLATE1Uncoated MicroplateSDS

  • RABQUENCHQuenching Solution for Cell-based ELISA Assay (Item E)SDS

  • RABSTOP1Stop SolutionSDS

  • RABTMB1TMB Substrate Reagent (Item J)SDS

  • RABWASH120X Wash Buffer Concentrate ASDS

  • RABWASH220X Wash Buffer Concentrate BSDS

See All (10)

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3


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