RAB0350

Sigma-Aldrich

Cell-Based ELISA for detecting phospho-ERK1 / 2 (pThr202 / pTyr204) in cultured cell lines

96 assays

NACRES:
NA.32

usage

96 assays

species reactivity

rat, human, mouse

application(s)

ELISA: suitable
capture ELISA: suitable

shipped in

wet ice

storage temp.

−20°C

General description

The Cell-Based ERK1/2 (pThr202 / pTyr204) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of ERK1/2 (pThr202 / pTyr204) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.

Immunogen

ERK1/2 (pThr202 / pTyr204) synthetic phosphopeptide

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABBLOCK5x Blocking Solution (Item F)

  • RABFIX1Fixing Solution (Item D)

  • RABHRP1HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1)

  • RABHRP2HRP-conjugated Anti-Mouse IgG Concentrate (Item I2)

  • RABPLATE1Uncoated Microplate

  • RABQUENCHQuenching Solution for Cell-based ELISA Assay (Item E)

  • RABSTOP1Stop Solution

  • RABTMB1TMB Substrate Reagent (Item J)

  • RABWASH120X Wash Buffer Concentrate A

  • RABWASH220X Wash Buffer Concentrate B

See All (10)

Signal Word

Danger

hazcat

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

storage_class_code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 3

Certificate of Analysis

Certificate of Origin

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