RAB0365

Human MMP-2 ELISA Kit

for serum, plasma, cell culture supernatant

• Synonym(s): MMP-2, Matrix metalloproteinase-2
• UNSPSC Code: 41116158
• NACRES: NA.32

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable; capture ELISA: suitable
• input: sample type cell culture supernatant(s); sample type plasma; sample type serum
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 3,500 pg/mL; standard curve range: 3.3-800 ng/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... MMP2(4313)

Description

General description

The Human MMP-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP-2 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human MMP2

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human MMP-2 ELISA kit has been used for the determination of MMP-2 in serum samples and cell supernatant.[1][2]

Human MMP-2 ELISA Kit has been used in ELISA (enzyme-linked immunosorbent assay).[3][4]

Biochem/physiol Actions

Matrix metalloproteinases (MMPs) enzymes play a central role in the maintenance and remodeling of the extracellular matrix.[5] Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, which has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis.[6][7][8][9] High levels of MMP-2 are observed in the acute phase of transverse myelitis.[10] It is upregulated in colorectal carcinoma and is associated with poor survival outcome.[11] MMP-2 gene haplotypes are also linked with risk of thoracic aortic dissection, a cardiovascular disorder with high death rate.[12]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABELADE ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 8B - Non-combustible corrosive hazardous materials
• flash_point_f: Not applicable
• flash_point_c: Not applicable