RAB0444

Cell-Based ELISA for detecting phospho-STAT3 (pTyr⁷⁰⁵) in cultured cell lines

96 assays

• NACRES: NA.32

Properties

• usage: 96 assays
• species reactivity: rat, human, mouse
• technique(s): ELISA: suitable; capture ELISA: suitable
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... STAT3(6774) ; mouse ... STAT3(20848) ; rat ... STAT3(25125)

Description

General description

The Cell-Based Stat3 (pTyr⁷⁰⁵) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of Stat3 (pTyr⁷⁰⁵) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.
The STAT3 (signal transducer and activator of transcription)gene is mapped to human chromosome 17q21.2. Upon phosphorylation, STAT3 translocates to nucleus from cytoplasm, where it mediates a number of biological processes. The protein structure includes the following domains : N-domain, coiled-coil domain, DNA-binding domain, linker domain, SH2 domain and transcriptional activation domain.

Immunogen

Stat3 (pTyr⁷⁰⁵) synthetic phosphopeptide

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Signal transducer and activator of transcription (STAT) proteins are a family of latent cytoplasmic transcription factors involved in cytokine, hormone, and growth factor signal transduction. STAT proteins mediate broadly diverse biological processes, including cell growth, differentiation, apoptosis, fetal development, transformation, inflammation, and immune response. Unlike all other members of the STAT gene family, ablation of STAT3 leads to embryonic lethality and it has been suggested that this protein might represent a primordial STAT protein. It evokes a number of distinct responses in different cells, including induction of an acute-phase response in hepatoma cells, stimulation of proliferation in B lymphocytes, activation of terminal differentiation and growth arrest in monocytes, and maintenance of the pluripotency of embryonic stem cells. Dysregulation of STAT3 signaling has been demonstrated to contribute to malignant cellular transformation. Src kinase-mediated activation of STAT3 has been shown to be essential in prostate and ovarian carcinomas. In head and neck cancers, constitutive STAT3 activity with up-regulated epidermal growth factor receptor (EGFR) signaling plays an important role in malignant proliferation. STAT3 may be a key player in the pathogenesis of diverse human cancers which makes this molecule a prime target for novel therapies.

Kit Components Also Available Separately

• RABBLOCK 5x Blocking Solution (Item F)
• RABFIX1 Fixing Solution (Item D)
• RABHRP1 HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1)
• RABHRP2 HRP-conjugated Anti-Mouse IgG Concentrate (Item I2)
• RABPLATE1 Uncoated Microplate
• RABQUENCH Quenching Solution for Cell-based ELISA Assay (Item E)
• RABSTOP1 Stop Solution
RABTMB1 TMB Substrate Reagent (Item J)
RABWASH1 20X Wash Buffer Concentrate A
RABWASH2 20X Wash Buffer Concentrate B

See All (10)

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3