RAB0507

Human VEGF-A ELISA Kit

for serum, plasma, cell culture supernatants and urine

• Synonym(s): VEGF-A, Vascular endothelial growth factor A
• UNSPSC Code: 41116158
• NACRES: NA.32

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable; capture ELISA: suitable
• input: sample type plasma; sample type cell culture supernatant(s); sample type serum; sample type urine
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 10 pg/mL; standard curve range: 8.23-6000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... VEGFA(7422)

Description

General description

The Human VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human VEGF in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human VEGF-A

Application

For research use only. Not for use in diagnostic procedures.
Human VEGF (vascular endothelial growth factor) ELISA Kit has been used to measure VEGFA levels in human umbilical vascular endothelial cells (HUVECs) incubated with plasma from pre-eclamptic and healthy pregnant women.[1][2]

Human VEGF-A ELISA Kit has been used to qualify:

• VEGF in human liver hepatoma cell line PLC-PRF-5[3],
• residual cytokines in decellularized meningeal scaffolds (DMS)[4]
• human periodontal ligament (hPDL) fibroblasts[5]

Biochem/physiol Actions

VEGF (vascular endothelial growth factor) is a potent angiogenic cytokine. It stimulates proliferation and migration of endothelial cells. VEGF induces microvascular permeability by enhancing function of vesicular-vacuolar organelle (VVO). VEGF plays a prominent role in both normal and pathological angiogenesis.[6] Substantial evidence suggests that VEGF is involved in the induction of tumor metastasis and intraocular neovascular syndromes.[7][8] Increased expression of VEGF causes retinal angiogenesis, thus, inhibition of VEGF using soluble VEGF-receptor chimeric proteins can be considered as a potential therapy for ischemic retinal disease.[8] Polymorphism in VEGF at C (-634) G is linked with diabetic retinopathy in type 2 diabetes. Altered expression of VEGF is also associated with rheumatoid arthritis, Graves′ disease, psoriasis, cardiovascular disease, and peripheral limb disease.[7]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABELADA ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
• RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 8A - Combustible corrosive hazardous materials