This kit has been optimized with cell lysate and tissue lysate as the input sample types. If you are interested in a kit that can be used for cell culture supernatants, we recommend RAB0507 which is designed for serum, plasma, cell culture supernatants and urine samples.
RAB0508
Human VEGF ELISA Kit
for cell and tissue lysates
Synonym(s):
VEGF-A, Vascular endothelial growth factor A
Sign In to View Organizational & Contract Pricing.
Select a Size
1 KIT
$608.00
$608.00
Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)
About This Item
NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... VEGFA(7422)
Input:
sample type cell lysate
sample type tissue lysate
sample type tissue lysate
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
Skip To
species reactivity
human
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, capture ELISA: suitable
input
sample type cell lysate
sample type tissue lysate
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... VEGFA(7422)
1 of 4
This Item | |||
|---|---|---|---|
| species reactivity human | species reactivity human | species reactivity human | species reactivity human |
| Gene Information human ... VEGFA(7422) | Gene Information human ... VEGFA(7422) | Gene Information human ... FLT4(2324) | Gene Information human ... KDR(3791) |
| assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% |
| input sample type cell lysate | input sample type plasma | input sample type serum | input sample type plasma |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
| technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable |
General description
The Human VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human VEGF in cell lysate and tissue lysate.
Immunogen
Recombinant Human VEGF-A
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
VEGF (vascular endothelial growth factor) is a potent angiogenic cytokine. It stimulates proliferation and migration of endothelial cells. VEGF induces microvascular permeability by enhancing function of vesicular-vacuolar organelle (VVO). VEGF plays a prominent role in both normal and pathological angiogenesis. Substantial evidence suggests that VEGF is involved in the induction of tumor metastasis and intraocular neovascular syndromes. Increased expression of VEGF causes retinal angiogenesis, thus, inhibition of VEGF using soluble VEGF-receptor chimeric proteins can be considered as a potential therapy for ischemic retinal disease. Polymorphism in VEGF at C (-634) G is linked with diabetic retinopathy in type 2 diabetes. Altered expression of VEGF is also associated with rheumatoid arthritis, Graves′ disease, psoriasis, cardiovascular disease, and peripheral limb disease.
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
Kit Components Also Available Separately
Product No.
Description
SDS
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
10 - Combustible liquids
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Miyako Kurihara-Shimomura et al.
International journal of oncology, 56(5), 1093-1100 (2020-04-23)
Mast cells (MCs) are present in the tumor stroma, and MCs that express the mast cell‑specific proteases tryptase and chymase (MCTC) exhibit several tumor‑related functions. It was previously reported that melanoma inhibitory activity (MIA) gene family members, including MIA, MIA2
Piyush Gondaliya et al.
PloS one, 13(11), e0208044-e0208044 (2018-11-30)
The role of DNA methylation has not been enough explored in pathophysiology of diabetic nephropathy (DN). However, according to recent reports it has been inferred that hypermethylation could be one of the principle cause associated with the enhancement of DN.
A common polymorphism in the 5'-untranslated region of the VEGF gene is associated with diabetic retinopathy in type 2 diabetes.
Awata T
Diabetes, 51, 1635-1639 (2002)
Suppression of retinal neovascularization in vivo by inhibition of vascular endothelial growth factor (VEGF) using soluble VEGF-receptor chimeric proteins.
Aiello LP
Proceedings of the National Academy of Sciences of the USA, 92, 10457-10461 (1995)
Vascular permeability factor/vascular endothelial growth factor: a multifunctional angiogenic cytokine.
Brown LF
EXS, 79, 233-269 (1997)
Related Content
Instructions
-
can this kit be used for cell culture supernatants as well? if the same sensitivity is required and if the lysis buffer is not used...
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service