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RAB0602

Sigma-Aldrich

Human AFP / Alpha-fetoprotein ELISA Kit

for serum, plasma, cell culture supernatants and urine

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NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type urine
sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 7.373-1800 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... AFP(174)

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Human SDF 1 α ELISA Kit

Gene Information

human ... AFP(174)

Gene Information

human ... CXCL12(6387)

Gene Information

human ... APOC1(341)

Gene Information

human ... APOE(348)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 7.373-1800 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 93.75-6000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 3.2-50000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.4 ng/mL
standard curve range: 0.41-100 ng/mL

input

sample type urine
sample type plasma
sample type serum
sample type cell culture supernatant(s)

input

sample type cell culture supernatant
sample type urine
sample type plasma

input

sample type urine
sample type serum
sample type cell culture supernatant(s)
sample type plasma

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The AFP ELISA kit provides for the quantitative measurement of Alpha-fetoprotein in Cell Culture Supernatants, Plasma and Serum.

Application

Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Human α-fetoprotein (AFP) ELISA kit has been used to detect levels of AFP in comparison with photoelectrochemical (PEC) immunosensor.

Biochem/physiol Actions

α-fetoprotein (AFP) facilitates the regulation of fatty acids in both fetal and proliferating adult liver cells. Overexpression of the gene has been observed in acute liver injuries, indicating active liver regeneration. AFP has been associated with fatty liver disease (FLD), a disease that may lead to cirrhosis and hepatocellular carcinoma. It functions as a tumor marker for HCC (hepatocellular carcinoma).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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25G
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