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RDD002

Sigma-Aldrich

HEPES

anhydrous, free-flowing, Redi-Dri, ≥99.5%

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Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

anhydrous

Quality Level

product line

Redi-Dri

Assay

≥99.5%

form

crystalline powder

quality

free-flowing

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 500 mg/mL, clear, colorless

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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This Item
54457H7523H6147
HEPES anhydrous, free-flowing, Redi-Dri™, ≥99.5%

Sigma-Aldrich

RDD002

HEPES

-
HEPES BioUltra, for molecular biology, ≥99.5% (T)

Sigma-Aldrich

54457

HEPES

Premium Grade
HEPES BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Sigma-Aldrich

H7523

HEPES

Premium Grade
HEPES BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

Sigma-Aldrich

H6147

HEPES

Premium Grade
product line

Redi-Dri

product line

BioUltra

product line

BioXtra

product line

BioXtra

assay

≥99.5%

assay

≥99.5% (T)

assay

≥99.5% (titration)

assay

≥99.5% (titration)

form

crystalline powder

form

powder

form

powder

form

powder

pH

5.0-6.5 (25 °C, 238 g/L)

pH

5.0-6.5 (25 °C, 1 M in H2O)

pH

5.0-6.5 (1 M in H2O)

pH

5.0-6.5 (25 °C, 238 g/L)

solubility

water: 500 mg/mL, clear, colorless

solubility

H2O: 1 M at 20 °C, clear, colorless

solubility

H2O: 1 M, clear, colorless

solubility

water: 500 mg/mL, clear, colorless

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
  • In the novel vesicle-budding protocol as a component in stock solution and buffer solutions
  • As a component in intracellular solution for the preparation of brain-slice recording
  • As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification
  • To maintain the pH at 7 in thermal denaturation assay of purified protein
  • In isocratic elution of protein using gel filtration
  • Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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T1503
Product Number
-
25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

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  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

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HEPES sodium salt

Cell-free Generation of COPII-coated Procollagen I Carriers.
Yuan L, et al.
Bio-protocol, 7(22) (2017)
Discovery and characterization of a prevalent human gut bacterial enzyme sufficient for the inactivation of a family of plant toxins.
Koppel N, et al.
eLife, 7, e33953-e33953 (2018)
Optogenetic Mapping of Synaptic Connections in Mouse Brain Slices to Define the Functional Connectome of Identified Neuronal Populations.
Mingote S, et al.
The Journal of Neuroscience (2015)
Assessing the corrosion of biodegradable magnesium implants: a critical review of current methodologies and their limitations.
Kirkland NT, et al.
Acta Biomaterialia, 8(3), 925-936 (2012)
Hydrogen ion buffers for biological research.
Good NE, et al.
Biochemistry, 5(2), 467-477 (1966)

Articles

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