RDD002

Sigma-Aldrich

HEPES

anhydrous, free-flowing, Redi-Dri, ≥99.5%

Synonym(s):
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein/REAXYS Number:
883043
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

grade

anhydrous

product line

Redi-Dri

assay

≥99.5%

form

crystalline powder

quality

free-flowing

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8 - 8.2

pKa (25 °C)

7.5

solubility

water: 500 mg/mL, clear, colorless

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
  • In the novel vesicle-budding protocol as a component in stock solution and buffer solutions
  • As a component in intracellular solution for the preparation of brain-slice recording
  • As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification
  • To maintain the pH at 7 in thermal denaturation assay of purified protein
  • In isocratic elution of protein using gel filtration
  • Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy

Packaging

2.5 kg in poly drum
1 kg in poly bottle

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Cell-free Generation of COPII-coated Procollagen I Carriers.
Yuan L, et al.
Bio-protocol, 7(22) (2017)
Discovery and characterization of a prevalent human gut bacterial enzyme sufficient for the inactivation of a family of plant toxins.
Koppel N, et al.
eLife, 7, e33953-e33953 (2018)
Optogenetic Mapping of Synaptic Connections in Mouse Brain Slices to Define the Functional Connectome of Identified Neuronal Populations.
Mingote S, et al.
The Journal of Neuroscience (2015)
Assessing the corrosion of biodegradable magnesium implants: a critical review of current methodologies and their limitations.
Kirkland NT, et al.
Acta Biomaterialia, 8(3), 925-936 (2012)
Hydrogen ion buffers for biological research.
Good NE, et al.
Biochemistry, 5(2), 467-477 (1966)
Articles
Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.
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