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RDD008

Sigma-Aldrich

Trizma® base

anhydrous, free-flowing, Redi-Dri, ≥99.9%

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Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

anhydrous

Quality Level

description

aminopeptidase substrate

product line

Redi-Dri

Assay

≥99.9%

form

powder

quality

free-flowing

impurities

≤0.2% Water (Karl Fischer)

color

white

pH

10.5-12
7-9

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: soluble (Water < 0.2%)

absorption

≤0.05 at 290 nm at 40%

λ

(A290 UV absorbance < 0.05)
in 5 mM Tris; DNA (40% (w/w))

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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1 of 4

This Item
93352T6066T1503
Trizma&#174; base anhydrous, free-flowing, Redi-Dri&#8482;, &#8805;99.9%

Sigma-Aldrich

RDD008

Trizma® base

-
Trizma&#174; base &#8805;99.0% (T)

Sigma-Aldrich

93352

Trizma® base

-
Trizma&#174; base BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, &#8805;99.9% (titration)

Sigma-Aldrich

T6066

Trizma® base

Premium Grade
Trizma&#174; base Primary Standard and Buffer, &#8805;99.9% (titration), crystalline

Sigma-Aldrich

T1503

Trizma® base

-
product line

Redi-Dri

product line

BioChemika

product line

-

product line

-

assay

≥99.9%

assay

≥99.0% (T)

assay

≥99.9% (titration)

assay

≥99.9% (titration)

form

powder

form

crystalline

form

crystalline powder

form

crystalline

impurities

≤0.2% Water (Karl Fischer)

impurities

-

impurities

DNase, RNase, protease, none detected, Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested, ≤0.2% water (Karl Fischer)

impurities

-

color

white

color

-

color

-

color

-

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Tris base has been used:
  • To prepare Tris-HCl, which is used as a component of lysis buffer for protein characterization
  • In the preparation of Tris-buffered saline Tween-20 (TBST) buffer for Western blotting
  • In the preparation of TBS (Tris-buffered saline) that is used for dialysis to neutralize a protein
  • As a component of nuclei extraction buffer

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Bankston AN, et al.
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Top-down characterization of chicken core histones.
Wu H, et al.
Journal of Proteomics, 184, 34-38 (2018)
A Streamlined Method for the Preparation of Growth Factor-enriched Thermosensitive Hydrogels from Soft Tissue.
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