RES0703P-A7

SAFC

PIPES

PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production, suitable for cell culture

Synonym(s):
PIPES, Piperazine-N,N′-bis(2-ethanesulfonic acid), Piperazine-1,4-bis(2-ethanesulfonic acid), 1,4-Piperazinediethanesulfonic acid
Empirical Formula (Hill Notation):
C8H18N2O6S2
CAS Number:
Molecular Weight:
302.37
Beilstein/REAXYS Number:
817713
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21
Pricing and availability is not currently available.

Quality Level

biological source

synthetic

Agency/Method

non-compendial

product line

PharmaGrade

assay

≥99.0%

form

powder

application(s)

cell culture | mammalian: suitable

impurities

Endotoxin and microbial; tested

useful pH range

6.1 - 7.5

pKa (25 °C)

6.8

mp

>300 °C (lit.)

solubility

1 M NaOH: 5%, clear, colorless

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)

InChI key

IHPYMWDTONKSCO-UHFFFAOYSA-N

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General description

PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit sigma-aldrich.com/safc-pharmagrade.

Application

PIPES is a biological buffer often referred to as a “Good′s” buffer. The pKa of PIPES is 6.8 which makes it ideal for biological applications at physiological conditions. PIPES is considered to be non-toxic to culture cell lines and provides high-solution clarity at the typical buffer pH.

PIPES can be used in a wide array of biological applications, including as a component in media formulations. Specific applications include buffers for cell culture and diagnostic reagent production.

Packaging

Product is available in the following package sizes:
RES0703P-A701X: 100 gm container
RES0703P-A702X: 1 kg container
RES0703P-A704X: 10 kg container

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Leticia A Montoya et al.
Analytical chemistry, 86(12), 6032-6039 (2014-05-24)
Sulfhydryl-containing compounds, including thiols and hydrogen sulfide (H2S), play important but differential roles in biological structure and function. One major challenge in separating the biological roles of thiols and H2S is developing tools to effectively separate the reactivity of these...
M Le Hir
Enzyme, 45(4), 194-199 (1991-01-01)
An impurity, probably an anion, present in some batches of the buffer substances 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES), 2-morpholinoethane sulfonic acid (Mes) and piperazine-1,4-bis(2-ethane sulfonic acid (Pipes), activates the soluble 5'-nucleotidase from rat kidney. The affinity of the enzyme for 5'-IMP...
Veronique Adoue et al.
Molecular systems biology, 10, 754-754 (2014-10-19)
Most complex disease-associated genetic variants are located in non-coding regions and are therefore thought to be regulatory in nature. Association mapping of differential allelic expression (AE) is a powerful method to identify SNPs with direct cis-regulatory impact (cis-rSNPs). We used...
Nicholas Light et al.
Epigenetics, 9(9), 1238-1251 (2014-07-24)
Allele-specific (AS) assessment of chromatin has the potential to elucidate specific cis-regulatory mechanisms, which are predicted to underlie the majority of the known genetic associations to complex disease. However, development of chromatin landscapes at allelic resolution has been challenging since...
Kalina Makowiecki et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(32), 10780-10792 (2014-08-08)
Repetitive transcranial magnetic stimulation (rTMS) is increasingly used as a treatment for neurological and psychiatric disorders. Although the induced field is focused on a target region during rTMS, adjacent areas also receive stimulation at a lower intensity and the contribution...

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