PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production., suitable for cell culture

BICINE, N,N-Bis(2-hydroxyethyl)glycine
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
Beilstein/REAXYS Number:
EC Number:
MDL number:
PubChem Substance ID:
Pricing and availability is not currently available.

Quality Level

biological source




product line





cell culture | mammalian: suitable


Endotoxin and microbial; tested

useful pH range

7.6 - 9.0

pKa (25 °C)



suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string




InChI key


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General description

PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit


Recommended buffer for low temperature biochemical work. Used in the preparation of stable substrate solution for serum guanase determination. Spacer in plasma protein fractionation with Sephadex® by isotachophoresis.
Bicine is a biological buffer often referred to as a “Good′s” buffer. The pKa of Bicine is 8.3 which makes Bicine is a good candidate for biological applications that require pH control above physiological. Bicine buffers are commonly used in electrophoresis and protein separations. Bicine is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.

Bicine can be used in a wide array of biological applications including as a component in media formulations. Specific applications include buffers for electrophoresis, protein purification and diagnostic reagent production.


Product is available in the following package sizes:
RES1151B-B701X: 100 gm container
RES1151B-B702X: 1 kg container
RES1151B-B704X: 10 kg container

Legal Information

Sephadex is a registered trademark of Cytiva


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

R Nakon et al.
Science (New York, N.Y.), 221(4612), 749-750 (1983-08-19)
Metal-ion affinity (formation) constants were determined for two "Good's" buffers, N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES) and N,N-bis(2-hydroxyethyl)glycine (bicine). The metal chelates formed undergo loss of an internal ligand (alcohol) proton (bicine) and undergo hydrolysis (bicine and TES) and dimerization reactions (TES). Bicine...
E Gopinath et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(9), 3041-3044 (1989-05-01)
The nature and rate of reduction of Hg2+ to Hg0 by 1,5-dihydro-3,(3-sulfopropyl)lumiflavin (FIH2) in buffered aqueous solutions (pH 4.7) is dependent on the ligation of Hg2+. In the presence of N,N-bis(2-hydroxyethyl)glycine or when ligated to ethylenediaminetetraacetic acid, the reduction is...
The myosin dimer: an intermediate in the self-assembly of the thick filament of vertebrate skeletal muscle.
J S Davis et al.
FEBS letters, 140(2), 293-297 (1982-04-19)
T A Churchill et al.
Transplantation, 65(4), 551-559 (1998-03-21)
This study was designed to investigate the effects of a modified University of Wisconsin (UW) solution supplemented with one of four buffering agents (histidine, bicine [N,N-bis(2-hydroxyethyl)glycine], tricine [N-tris(hydroxymethyl)methylglycine], and Tris) on liver metabolism during cold ischemic storage. Rat livers were...
Raewyn M Town et al.
The journal of physical chemistry. A, 112(12), 2563-2571 (2008-03-04)
The impact of ligand protonation on the complexation kinetics of higher-order complexes is quantitatively described. The theory is formulated on the basis of the usual situation for metal complex formation in aqueous systems in which the exchange of water for...

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