RES1151B-B7

SAFC

Bicine

PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production., suitable for cell culture

Synonym(s):
BICINE, N,N-Bis(2-hydroxyethyl)glycine
Empirical Formula (Hill Notation):
C6H13NO4
CAS Number:
Molecular Weight:
163.17
Beilstein/REAXYS Number:
1769362
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21
Pricing and availability is not currently available.

Quality Level

biological source

synthetic

Agency/Method

non-compendial

product line

PharmaGrade

form

powder

application(s)

cell culture | mammalian: suitable

impurities

Endotoxin and microbial; tested

useful pH range

7.6 - 9.0

pKa (25 °C)

8.3

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

OCCN(CCO)CC(O)=O

InChI

1S/C6H13NO4/c8-3-1-7(2-4-9)5-6(10)11/h8-9H,1-5H2,(H,10,11)

InChI key

FSVCELGFZIQNCK-UHFFFAOYSA-N

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General description

PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit sigma-aldrich.com/safc-pharmagrade.

Application

Recommended buffer for low temperature biochemical work. Used in the preparation of stable substrate solution for serum guanase determination. Spacer in plasma protein fractionation with Sephadex® by isotachophoresis.
Bicine is a biological buffer often referred to as a “Good′s” buffer. The pKa of Bicine is 8.3 which makes Bicine is a good candidate for biological applications that require pH control above physiological. Bicine buffers are commonly used in electrophoresis and protein separations. Bicine is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.

Bicine can be used in a wide array of biological applications including as a component in media formulations. Specific applications include buffers for electrophoresis, protein purification and diagnostic reagent production.

Packaging

Product is available in the following package sizes:
RES1151B-B701X: 100 gm container
RES1151B-B702X: 1 kg container
RES1151B-B704X: 10 kg container

Legal Information

Sephadex is a registered trademark of Cytiva

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

R Nakon et al.
Science (New York, N.Y.), 221(4612), 749-750 (1983-08-19)
Metal-ion affinity (formation) constants were determined for two "Good's" buffers, N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES) and N,N-bis(2-hydroxyethyl)glycine (bicine). The metal chelates formed undergo loss of an internal ligand (alcohol) proton (bicine) and undergo hydrolysis (bicine and TES) and dimerization reactions (TES). Bicine...
E Gopinath et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(9), 3041-3044 (1989-05-01)
The nature and rate of reduction of Hg2+ to Hg0 by 1,5-dihydro-3,(3-sulfopropyl)lumiflavin (FIH2) in buffered aqueous solutions (pH 4.7) is dependent on the ligation of Hg2+. In the presence of N,N-bis(2-hydroxyethyl)glycine or when ligated to ethylenediaminetetraacetic acid, the reduction is...
The myosin dimer: an intermediate in the self-assembly of the thick filament of vertebrate skeletal muscle.
J S Davis et al.
FEBS letters, 140(2), 293-297 (1982-04-19)
T A Churchill et al.
Transplantation, 65(4), 551-559 (1998-03-21)
This study was designed to investigate the effects of a modified University of Wisconsin (UW) solution supplemented with one of four buffering agents (histidine, bicine [N,N-bis(2-hydroxyethyl)glycine], tricine [N-tris(hydroxymethyl)methylglycine], and Tris) on liver metabolism during cold ischemic storage. Rat livers were...
Raewyn M Town et al.
The journal of physical chemistry. A, 112(12), 2563-2571 (2008-03-04)
The impact of ligand protonation on the complexation kinetics of higher-order complexes is quantitatively described. The theory is formulated on the basis of the usual situation for metal complex formation in aqueous systems in which the exchange of water for...

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