RES1161B-A7

SAFC

BIS-TRIS

PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production., suitable for cell culture

Synonym(s):
2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol
Empirical Formula (Hill Notation):
C8H19NO5
CAS Number:
Molecular Weight:
209.24
Beilstein/REAXYS Number:
2205275
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21
Pricing and availability is not currently available.

biological source

synthetic

Agency/Method

non-compendial

product line

PharmaGrade

form

powder

application(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals, tested

useful pH range

5.8 - 7.2

pKa (25 °C)

6.5

solubility

H2O: 0.1 M, clear, colorless (aqueous)

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

InChI key

OWMVSZAMULFTJU-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit sigma-aldrich.com/safc-pharmagrade.

Application

Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological based formulations.

Bis Tris is used in a wide variety of biological applications including culture media, electrophoresis separations and diagnostic reagent formulations.

Packaging

Product is available in the following package sizes:
RES1161B-A701X: 100g container
RES1161B-A702X 1kg container
RES1161B-A704X: 10kg container
RES1161B-A705X: 25kg container

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Codes

Xi

Risk Statement

36/37/38

Safety Statement

26-36

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Alastair W Skeffington et al.
Plant physiology, 165(2), 866-879 (2014-05-02)
The first step on the pathway of starch degradation in Arabidopsis (Arabidopsis thaliana) leaves at night is the phosphorylation of starch polymers, catalyzed by glucan, water dikinase (GWD). It has been suggested that GWD is important for the control of...
Zahra Sabouri et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(25), E2567-E2575 (2014-05-14)
The best-understood mechanisms for achieving antibody self/non-self discrimination discard self-reactive antibodies before they can be tested for binding microbial antigens, potentially creating holes in the repertoire. Here we provide evidence for a complementary mechanism: retaining autoantibodies in the repertoire displayed...
Elizabeth M MacDonald et al.
Disease models & mechanisms, 7(4), 471-481 (2014-02-08)
The purpose of our study was to compare two acquired muscle atrophies and the use of myostatin inhibition for their treatment. Myostatin naturally inhibits skeletal muscle growth by binding to ActRIIB, a receptor on the cell surface of myofibers. Because...
Lesley Cheng et al.
Kidney international, 86(2), 433-444 (2013-12-20)
Micro RNAs (miRNAs) have been shown to circulate in biological fluids and are enclosed in vesicles such as exosomes; they are present in urine and represent a noninvasive methodology to detect biomarkers for diagnostic testing. The low abundance of RNA...
Shirya Rashid et al.
Circulation, 130(5), 431-441 (2014-07-30)
Proprotein convertase subtilisin kexin type 9 (PCSK9) promotes the degradation of the low-density lipoprotein (LDL) receptor (LDLR), and its deficiency in humans results in low plasma LDL cholesterol and protection against coronary heart disease. Recent evidence indicates that PCSK9 also...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.