TRIS hydrochloride

Trizma® hydrochloride, TRIS HCl, Tris(hydroxymethyl)aminomethane hydrochloride, TRIS hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
Beilstein/REAXYS Number:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

biological source





cell culture | mammalian: suitable


Endotoxin, microbial, and trace metals, tested

useful pH range

7.0 - 9.0

pKa (25 °C)



suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string




InChI key


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The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Tris Hydrochloride is a commonly used biological buffer which is also referred to as Trizma HCl. Tris as a buffer has very broad usage in biological systems such as diagnostics, downstream purification and cell culture. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.

Tris HCl s used in biopharmaceutical buffer formulations (both upstream and downstream), electrophoresis separations and diagnostic reagent formulations. Tris HCl is especially useful in downstream purification processes.


Product is available in the following package sizes:
RES3098T-B701X: 100g container
RES3098T-B703X: 5kg container
RES3098T-B705X: 25kg container
RES3098T-B706X: 50kg container

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC


13 - Non Combustible Solids

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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