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RES3098T-B7

SAFC

TRIS hydrochloride

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Synonym(s):
Trizma® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein/REAXYS Number:
3675235
EC Number:
MDL number:
NACRES:
NA.21

biological source

synthetic

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals, tested

useful pH range

7.0-9.0

pKa (25 °C)

8.1

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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1 of 4

This Item
93363T5941RDD009
vibrant-m

RES3098T-B7

TRIS hydrochloride

-
vibrant-m

93363

Trizma® hydrochloride

Premium Grade
vibrant-m

T5941

Trizma® hydrochloride

Premium Grade
vibrant-m

RDD009

Trizma® hydrochloride

-
foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

foreign activity

-

foreign activity

-

foreign activity

-

form

powder

form

powder or crystals

form

crystalline powder

form

powder

impurities

Endotoxin, microbial, and trace metals, tested

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

impurities

DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected, endotoxin and total aerobic microbial count, tested, ≤0.5% water (Karl Fischer)

impurities

≤0.5% water (Karl Fischer)

suitability

suitable for manufacturing use

suitability

-

suitability

-

suitability

-

biological source

synthetic

biological source

-

biological source

-

biological source

-

Application

Tris Hydrochloride is a commonly used biological buffer which is also referred to as Trizma HCl. Tris as a buffer has very broad usage in biological systems such as diagnostics, downstream purification and cell culture. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.

Tris HCl s used in biopharmaceutical buffer formulations (both upstream and downstream), electrophoresis separations and diagnostic reagent formulations. Tris HCl is especially useful in downstream purification processes.

Packaging

Product is available in the following package sizes:
RES3098T-B701X: 100g container
RES3098T-B703X: 5kg container
RES3098T-B705X: 25kg container
RES3098T-B706X: 50kg container

Other Notes

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_c

Not applicable


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Tris base =99.9% (titration), abs., 100 mg/mL, 300 nm ≤0.015

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TRIS-RO

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Millipore

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TRIS Hydrochloride

Tromethamine

SAFC

RES3193T-A7

Tromethamine

Sigma-Aldrich

Sigma-Aldrich

12-457

MYPT1 (654-880)

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