MilliporeSigma
All Photos(2)

Documents

RES6007H-A7

SAFC

HEPES sodium salt

All Photos(2)
Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
75277-39-3
Molecular Weight:
260.29
EC Number:
278-169-7
MDL number:
MFCD00036463
NACRES:
NA.21

biological source

synthetic

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals; tested

useful pH range

6.8-8.2

pKa (25 °C)

7.5

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
RDD035H3784H7006
HEPES sodium salt

SAFC

RES6007H-A7

HEPES sodium salt

HEPES sodium salt ≥99.5% (titration), free-flowing, Redi-Dri™

Sigma-Aldrich

RDD035

HEPES sodium salt

HEPES sodium salt BioPerformance Certified, suitable for cell culture, ≥99.0%

Sigma-Aldrich

H3784

HEPES sodium salt

HEPES sodium salt ≥99.0% (titration)

Sigma-Aldrich

H7006

HEPES sodium salt

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

impurities

Endotoxin, microbial, and trace metals; tested

impurities

-

impurities

endotoxin and total aerobic microbial count, tested

impurities

-

suitability

suitable for manufacturing use

suitability

-

suitability

-

suitability

-

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

foreign activity

-

foreign activity

DNase, RNase, NICKase, protease, none detected

foreign activity

-

Quality Level

400

Quality Level

100

Quality Level

400

Quality Level

400

General description

Our SAFC® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.

M-Clarity Program

Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.

Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

Application

HEPES Sodium is a biological buffer often referred to as a “Good′s” buffer. HEPES Sodium, used in conjunction with HEPES, is the most commonly used biological buffer formulation since the pKa of the buffer is 7.31 which corresponds to the physiological pH of 7.36. HEPES Sodium is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.

HEPES Sodium is used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagent formulations.

Packaging

The product is available in the following package sizes:
RES6007H-A701X: 100g container
RES6007H-A702X: 1kg container
RES6007H-A704X: 10kg container
RES6007H-A705X: 25kg container

Legal Information

SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 6

1 of 6

MOPS

SAFC

PHG0007

MOPS

HEPES sodium salt solution 1M, BioReagent, suitable for cell culture

Sigma-Aldrich

H3662

HEPES sodium salt solution

MOPS sodium salt

SAFC

RES0197M-A7

MOPS sodium salt

SAFC

SAFC

14324C

EX-CELL® 302 Serum-Free Medium for CHO Cells

HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

Sigma-Aldrich

H4034

HEPES

Calcium lactate pentahydrate meets USP testing specifications

Sigma-Aldrich

C8356

Calcium lactate pentahydrate

Anca G Delgado et al.
Microbial cell factories, 11, 128-128 (2012-09-15)
Buffering to achieve pH control is crucial for successful trichloroethene (TCE) anaerobic bioremediation. Bicarbonate (HCO3-) is the natural buffer in groundwater and the buffer of choice in the laboratory and at contaminated sites undergoing biological treatment with organohalide respiring microorganisms.
Peng Yang
Macromolecular bioscience, 12(8), 1053-1059 (2012-06-19)
When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue"
Tina Vognsen et al.
Biochemical and biophysical research communications, 420(1), 188-192 (2012-03-15)
The crystal structure of the NTF2-like domain of the Drosophila homolog of Ras GTPase SH3 Binding Protein (G3BP), Rasputin, was determined at 2.7Å resolution. The overall structure is highly similar to nuclear transport factor 2: It is a homodimer comprised
S Hugel et al.
British journal of pharmacology, 166(8), 2402-2416 (2012-03-29)
Artificial buffers such as HEPES are extensively used to control extracellular pH (pH(e) ) to investigate the effect of H(+) ions on GABA(A) receptor function. In neurones cultured from spinal cord dorsal horn (DH), dorsal root ganglia (DRG) and cerebellar
Jihye Park et al.
The Analyst, 137(19), 4411-4414 (2012-08-07)
We have developed a quickly responsive, and specific fluorescent assay for the detection of Au(III) on the basis of the formation of gold nanoparticles in the presence of HEPES, which cleave the C-I bond of I-BODIPY 1 to yield the
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service