RES6008H-A7

SAFC

HEPES, Hemisodium Salt

PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production, suitable for cell culture

Synonym(s):
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) hemisodium salt, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt, HEPES hemisodium salt
Empirical Formula (Hill Notation):
C8H17.5N2O4S · 0.5Na
CAS Number:
Molecular Weight:
249.30
NACRES:
NA.21
Pricing and availability is not currently available.

biological source

synthetic

Agency/Method

non-compendial

product line

PharmaGrade

assay

≥96.0% NaOH basis (Reverse titration)

form

powder

application(s)

cell culture | mammalian: suitable

impurities

Endotoxin and microbial; tested

useful pH range

6.8 - 8.2

pKa 

7.5

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

Na+.OCCN1CCN(CC1)CCS(O-)(=O)=O

General description

PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit sigma-aldrich.com/safc-pharmagrade.

Application

HEPES Hemisodium is a biological buffer often referred to as a “Good′s” buffer. As the hemisodium salt, this buffer can be used directly to prepare a working buffer solution of HEPES. This biological buffer formulation is ideal since the pKa of the buffer is 7.31 which corresponds to the physiological pH of 7.36. HEPES Hemisodium is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.

HEPES Hemisodium can be used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagent formulations.

Packaging

Product is available in the following package sizes:
RES6008H-A701X: 100 gm container
RES6008H-A702X: 1 kg container
RES6008H-A704X: 10 kg container

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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