MilliporeSigma
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REV888

Fire Monkey / Fire Flower High Molecular Weight-DNA Extraction Kit

Synonym(s):
long-read DNA sequencing, High molecular weight DNA extraction, nulcleic acid purification kit

feature

Includes Columns in 2 mL Collection Tubes, Lysis Solution DNA, Binding Solution, Wash Solution, Elution Buffer

packaging

kit of sample (10 extractions)
kit of 1 ea (50 extractions)

storage temp.

room temp

General description

For the extraction of high molecular weight DNA from animal and bacterial cells, and size selection of extracted DNA from all sample types.

FIRE MONKEY - HMW DNA EXTRACTION
Fire Monkey is a standard spin-column process which extracts HMW-DNA with average strand lengths of 100kb and above from both bacterial and mammalian cells within 1hr.

FIRE FLOWER - DNA SIZE SELECTION KIT
Fire Flower is a standard spin-column process that can size select extracted DNA from all sample sources within 15mins. Fire Flower can increase the overall average strand length by 30%, while depleting DNA fragments up to 30kb. Fire Flower can be used to size-select up to 200 μL of extracted DNA despite the sample being rich in small DNA fragments.


The two major disruptive features of Fire Monkey are that firstly, it generates average DNA fragment lengths of between 100kbp – 130kbp and secondly, it significantly reduces the amount of small DNA fragments shorter than 10kbp in the final product. This combination, where significant amounts of the DNA fragments have intact lengths of over 165,000 base pairs and up to several hundred thousands base pairs in length, combined with the absence of most of the smaller DNA fragments, through its inbuilt size selection capability, massively improves the eventual long-read sequencing of the sample. Fire Monkey improves the N50 measure of sequencing quality by 100% and yet it takes a sixth of the time to do so compared to the main competing NAIP kits.

Features and Benefits

  • Protocols are user-friendly and can be high-throughput.
  • Only basic laboratory equipment is required
  • Extract and purify long-fragments of DNA within one hour
  • In-built size-exclusion limits fragments <10kb

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

Quotes and Ordering

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