Skip to Content
MilliporeSigma
All Photos(4)

Documents

S0677

Sigma-Aldrich

Streptavidin from Streptomyces avidinii

recombinant, expressed in E. coli, lyophilized powder

Sign Into View Organizational & Contract Pricing

CAS Number:
MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

recombinant

expressed in E. coli

Quality Level

form

lyophilized powder

capacity

4 mol/mol binding capacity (Biotin)

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

Streptavidin is a biotin-binding protein that is obtained from Streptomyces avidinii. It is resistant to extreme temperature, pH, detergents and proteolytic enzymes. Streptavidin has a molecular weight of 60kDa and exists as a homotetramer.

Application

Streptavidin from Streptomyces avidinii has been used-
  • for blocking of the AFM (atomic force microscopy) tip, during the measurement of unbinding forces in the avidin-biotin system
  • for coupling of anti-HA (hemagglutinin) antibodies during cell free synthesis and assembly of proteins on a biochip
  • for the coating of the gelatin flow phantoms to eventually determine the feasibility of IVUS (intravascular ultrasound) transducer for (biotinylated) microbubble-based drug delivery
  • to immobilize proteins and oligonucleotides

Biochem/physiol Actions

Streptavidin is an antibiotic that functions by binding to and depleting the essential vitamin biotin from the surrounding environment. Because of its unique properties, streptavidin has found various applications in biological studies, including immunotherapy, immunoassays, hybridization assays, lymphocyte activation, antigen localization and affinity chromatography.

Unit Definition

One unit will bind 1.0 μg biotin.

Physical form

Lyophilized from 5 mM potassium phosphate buffer

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Determination of binding curves via protein micropatterning in vitro and in living cells
Stefan S
Cytometry (2012)
Nicola Ilk et al.
Applied and environmental microbiology, 68(7), 3251-3260 (2002-06-29)
The nucleotide sequence encoding the crystalline bacterial cell surface (S-layer) protein SbpA of Bacillus sphaericus CCM 2177 was determined by a PCR-based technique using four overlapping fragments. The entire sbpA sequence indicated one open reading frame of 3,804 bp encoding
The properties of streptavidin, a biotin-binding protein produced by Streptomycetes
Louis C
Archives of Biochemistry and Biophysics, 106 (1964)
Simplified Paper Format for Detecting HIV Drug Resistance in Clinical Specimens by Oligonucleotide Ligation
Nuttada Panpradist
PLoS ONE (2016)
Identification of the Heparan Sulfate Binding Sites in the Cellular Prion Protein*
Richard G
The Journal of Biological Chemistry (2002)

Related Content

Access high-quality reagents for antibody workflows, including adjuvants, blocking reagents, conjugation kits, and more.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service