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MilliporeSigma

S1402

Sigma-Aldrich

Sulforhodamine B sodium salt

powder, BioReagent, suitable for cell culture

Synonym(s):

Kiton Red 620

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About This Item

Empirical Formula (Hill Notation):
C27H29N2NaO7S2
CAS Number:
3520-42-1
Molecular Weight:
580.65
Colour Index Number:
45100
Beilstein/REAXYS Number:
3886008
EC Number:
222-529-8
MDL number:
MFCD00010180
UNSPSC Code:
12352207
PubChem Substance ID:
24899477
NACRES:
NA.75

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biological source

synthetic (organic)

Quality Level

100

product line

BioReagent

form

powder

composition

Dye content, ~75%

technique(s)

cell culture | mammalian: suitable

solubility

methanol: 1 mg/mL

fluorescence

λex 565 nm; λem 586 nm in H2O

SMILES string

[Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1

InChI

1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1

InChI key

SXQCTESRRZBPHJ-UHFFFAOYSA-M

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1 of 4

This Item
S901223016286186
Sulforhodamine B sodium salt powder, BioReagent, suitable for cell culture

Sigma-Aldrich

S1402

Sulforhodamine B sodium salt

Sulforhodamine B sodium salt Technical grade

Sigma-Aldrich

S9012

Sulforhodamine B sodium salt

Sulforhodamine B Dye content 75 %

Sigma-Aldrich

230162

Sulforhodamine B

Sulforhodamine B acid chloride suitable for fluorescence, technical

Sigma-Aldrich

86186

Sulforhodamine B acid chloride

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

microbe id | staining: suitable

technique(s)

-

fluorescence

λex 565 nm; λem 586 nm in H2O

fluorescence

-

fluorescence

-

fluorescence

λex 560 nm; λem 580 nm in chloroform, λex 560 nm; λem ~580 nm in chloroform

solubility

methanol: 1 mg/mL

solubility

-

solubility

methanol: 1 mg/mL

solubility

-

form

powder

form

powder

form

powder

form

powder

composition

Dye content, ~75%

composition

Dye content, ~75%

composition

Dye content, 75%

composition

-

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

General description

Sulforhodamine B is a rhodamine derived[1] aminoxanthene dye. It contains two sulfonic acids groups which separate in alkaline condition.[2]

Application

Sulforhodamine B sodium salt has been used:
  • for relative total cell mass quantification[3]
  • to investigate the viscosity of coacervates[4]
  • as a molecular rotor for microviscosity determination in aerosol droplets[5]

Biochem/physiol Actions

Sulforhodamine B sodium salt is useful to measure cell biomass and also for cytotoxicity screening.[6][2]

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBR3296
SHBN2223
SHBL9542
MKCD3873
MKCD1019

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Bioconjugate Techniques, 317-317 (1996)
Luca Giordano et al.
American journal of respiratory cell and molecular biology, 60(5), 515-522 (2018-10-20)
Cigarette smoke (CS) exposure is the predominant risk factor for the development of chronic obstructive pulmonary disease (COPD) and the third leading cause of death worldwide. We aimed to elucidate whether mitochondrial respiratory inhibition and oxidative stress are triggers in
Issues in Pharmacology, Pharmacy, Drug Research, and Drug Innovation (2012)
Inhibition of mitochondrial complex III blocks neuronal differentiation and maintains embryonic stem cell pluripotency
Pereira SL, et al.
PLoS ONE, 8(12), e82095-e82095 (2013)
Quantitative sensing of microviscosity in protocells and amyloid materials using fluorescence lifetime imaging of molecular rotors
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 8947, 89471C-89471C (2014)
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