S1451

Sigma-Aldrich

Anti-Sox2 antibody, Mouse monoclonal

clone SOX2-6, purified from hybridoma cell culture

Synonym(s):
Anti-SRY (sex determining region Y)-box 2, Anti-Sex-determining region Y-box 2, Anti-ANOP3, Anti-MCOPS3, Anti-SRY-related HMG-box gene 2
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

SOX2-6, monoclonal

form

buffered aqueous solution

mol wt

antigen ~35 kDa

species reactivity

human, mouse

concentration

~1.0 mg/mL

application(s)

western blot: 0.5-1.0 μg/mL using whole extract of human NT2 cells

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... SOX2(6657)
mouse ... Sox2(20674)
rat ... Sox2(499593)

General description

Monoclonal Anti-SOX2 (mouse IgG1 isotype) is derived from the hybridoma SOX2-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice.
SOX2 (sex-determining region Y-box 2) is a transcription factor that is made of 317 amino acid. It has a HMG (high mobility group box) domain and is a critical transcription regulator of normal stem cell. It is located on human chromosome 3q26.3.

Application

Anti-Sox2 antibody has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.
SOX2 (sex-determining region Y-box 2) down-regulation reduces the stem cell count, that affects the breast cancer cells in initiating tumor progression. It participates in chemoresistance and regular lung cancer therapies. In mouse, SOX2 plays an important role in branching morphogenesis and regulating lung epithelial cell differentiation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

CIBZ Regulates Mesodermal and Cardiac Differentiation of by Suppressing T and Mesp1 Expression in Mouse Embryonic Stem Cells
Kotoku T, et al.
Scientific Reports, 6 (2016)
Differential expression of ID4 and its association with TP53 mutation, SOX2, SOX4 and OCT-4 expression levels
Galatro TF, et al.
PLoS ONE, 8(4) (2013)
Yang Li et al.
International journal of molecular medicine, 37(4), 998-1004 (2016-03-05)
Phosphoglucose isomerase/autocrine motility factor (PGI/AMF) is secreted by tumors and influences tumor growth and metastasis. In order to investigate the effects of silencing PGI/AMF on the migration and the sphere forming abilities of human glioblastoma U87 cells, as well as...
SOX2 is an oncogene activated by recurrent 3q26.3 amplifications in human lung squamous cell carcinomas
Hussenet T, et al.
PLoS ONE, 5(1) (2010)
Mutations in SOX2 cause anophthalmia
Fantes J, et al.
Nature Genetics, 33(4), 462-462 (2003)
Articles
Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.
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