S1452

Sigma-Aldrich

MISSION® siRNA Transfection Reagent

Synonym(s):
MISSION siRNA Transfection Reagent
NACRES:
NA.51

Quality Level

product line

MISSION®

form

aqueous solution

shipped in

wet ice

storage temp.

2-8°C

General description

The MISSION® siRNA Transfection Reagent is for the transfection of siRNA to achieve ≥90% silencing efficiency for transient knockdown of eukaryotic gene expression.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.

Other Notes

Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Pictograms

Flame

Signal Word

Warning

Hazard Statements

RIDADR

UN1170 - class 3 - PG 3 - Ethanol, solution

WGK Germany

WGK 3

Flash Point(F)

120.2 °F

Flash Point(C)

49 °C

Tuyen T Dang et al.
Cancers, 12(7) (2020-07-11)
It was recently shown that the 5' to 3' exoribonuclease XRN2 is involved in the DNA damage response. Importantly, loss of XRN2 abrogates DNA double stranded break repair via the non-homologous end-joining pathway. However, the mechanistic details of how XRN2...
Ilaria Guerriero et al.
Oncotarget, 8(12), 19172-19191 (2016-11-24)
Hyperactivation of the PI3K/AKT pathway is observed in most human cancer including lung carcinomas. Here we have investigated the role of miRNAs as downstream targets of activated PI3K/AKT signaling in Non Small Cell Lung Cancer (NSCLC). To this aim, miRNA...
A E Witt et al.
Oncogene, 36(12), 1707-1720 (2016-10-04)
Tumours are comprised of a highly heterogeneous population of cells, of which only a small subset of stem-like cells possess the ability to regenerate tumours in vivo. These cancer stem cells (CSCs) represent a significant clinical challenge as they are...
Eirini Kefaloyianni et al.
Journal of the American Society of Nephrology : JASN, 30(12), 2370-2383 (2019-11-05)
Sustained activation of EGF receptor (EGFR) in proximal tubule cells is a hallmark of progressive kidney fibrosis after AKI and in CKD. However, the molecular mechanisms and particular EGFR ligands involved are unknown. We studied EGFR activation in proximal tubule...
Ponnuraj Nagendraprabhu et al.
PLoS pathogens, 13(12), e1006779-e1006779 (2017-12-16)
Poxviruses have evolved multiple strategies to subvert signaling by Nuclear Factor κB (NF-κB), a crucial regulator of host innate immune responses. Here, we describe an orf virus (ORFV) virion-associated protein, ORFV119, which inhibits NF-κB signaling very early in infection (≤...
Articles
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