Documents

S1576

Sigma-Aldrich

Anti-SDCBP (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-ST1, Anti-Pro-TGF-α−cytoplasmic domain-interacting protein 18, Anti-MDA-9, Anti-Syntenin, Anti-TACIP18, Anti-Melanoma differentiation associated protein-9, Anti-Syndecan binding protein (syntenin), Anti-SYCL
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~30 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

application(s)

immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells
indirect immunofluorescence: 10-20 μg/mL using human HeLa cells
western blot: 2-4 μg/mL using whole extracts of human HEK-293T cells

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... SDCBP(6386)

General description

Syndecan binding protein (SDCBP) is also named as syntenin and melanoma differentiation associated gene-9 (MDA-9) contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. SDCBP is located to membrane associated adherens junctions and focal adhesions. It is present in the endoplasmic reticulum and nucleus. SDCBP gene is mapped to human chromosome 8q12.1 and has 9 exons and is expressed in fetal kidney, liver, lung and brain.

Specificity

Anti-SDCBP (N-terminal) recognizes human SDCBP.

Application

Anti-SDCBP (N-terminal) antibody produced in rabbit may be used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Biochem/physiol Actions

Syndecan binding protein (SDCBP) might distress the organization of cytoskeletal-membrane and the stimulation of transcription factors. It may also affect cell adhesion and protein trafficking. SDCBP promotes metastasis in human melanoma cells by activating c-Src. Alternative splicing results in multiple transcript variants encoding different isoforms. Syntenin enables the recruitment of ubiquitinated proteins to its transmembrane partners by interacting with ubiquitin through is C- and N-terminal regions. It plays a key role in synapses formation and maturation. Upregulation of syntenin blocks human immunodeficiency virus-1 (HIV-1) production and HIV-mediated cell fusion. Depletion of this gene enhances the entry of HIV-1.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

1

Certificate of Analysis
Certificate of Origin
SDCBP (syndecan binding protein (syntenin))
Gangemi R, et al.
Atlas of Genetics and Cytogenetics in Oncology and Haematology (2020)
Tianzhen He et al.
Frontiers in immunology, 9, 1556-1556 (2018-07-25)
There is now compelling evidence that tumor necrosis factor (TNF) preferentially activates and expands CD4+Foxp3+ regulatory T cells (Tregs) through TNF receptor type II (TNFR2). However, it remains unclear which signaling transduction pathway(s) of TNFR2 is required for the stimulation...
Syndecan binding protein (SDCBP) is overexpressed in estrogen receptor negative breast cancers, and is a potential promoter for tumor proliferation
Qian XL, et al.
PLoS ONE, 8(3), e60046-e60046 (2013)

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