S1577

Sigma-Aldrich

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor human

recombinant, expressed in E. coli, lyophilized powder, ≥97% (SDS-PAGE), suitable for cell culture

Synonym(s):
SDF-1α, SDF-1, SDF1
NACRES:
NA.32

Quality Level

biological source

human

recombinant

expressed in E. coli

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

≤50 ng/mL EC50

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg protein Endotoxin

color

white

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CXCL12((6387)

General description

Stromal Cell-Derived Factor 1α (SDF-1α) or pre-B Cell Growth Stimulation Factor (PBSF) is an 8 kDa chemokine peptide. SDF-1 was identified using signal sequence trap cloning. With this method, cDNAs have been cloned using mouse and human stromal cell lines. This cytokine is widely accepted as a CXC chemokine of the intercrine-macrophage inflammatory protein superfamily. The gene has been mapped to chromosome 10q and is encoded by 4 exons. Expression of the human SDF1 gene is abundant in the pancreas, spleen, ovary, and small intestine. The presence of a GC-rich sequence and the lack of a TATA box in the 5∅-flanking region of the gene are consistent with ubiquitous expression.

Biochem/physiol Actions

SDF-1α functions as a pre-B cell growth factor in the presence of IL-7 (interleukin-7) and a potent chemoattractant for T-lymphocytes and monocytes. It has also been found to be a ligand for CXCR4 (chemokine receptor type 4) or the orphan receptor LESTR/fusin (putative chemokine receptor). By signaling through the receptor, SDF1 may serve as an inhibitor of HIV-1 (human immunodeficiency virus), which utilizes the LESTR/fusin receptor as a point of entry. The SDF-1/CXCR4 signalling pathway is associated with cancer cell proliferation, migration and survival. Overexpression of SDF1 is observed in breast carcinoma, ovarian carcinoma and papillary thyroid carcinoma. It enhances angiogenesis in the ischemic tissue. SDF1 offers CXCR4 mediated neuroprotection during post ischemic stroke.

Physical form

This product is lyophilized from a 0.2 μm-filtered solution containing 10 μg SDF-1α and 0.5 mg bovine serum albumin in PBS.

Reconstitution

Reconstitute the contents of the vial using sterile buffer or medium containing a minimum of 0.1% BSA or HSA to a stock concentration of ≥10 μg/ml. Additional filtration of the stock solution is not recommended as this may result in loss of product due to adsorption onto the filter membrane.

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Chemokines in multiple myeloma
Aggarwal R, et al.
Experimental Hematology, 34(10), 1289?-12295 (2006)
Diversity and inter-connections in the CXCR4 chemokine receptor/ligand family: molecular perspectives
Pawig L, et al.
Frontiers in Immunology (2015)
Wnt/?-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells
International Journal of Clinical and Experimental Pathology, 8(10), 12357?12367-12357?12367 (2015)
The diagnostic and prognostic value of serum CXCL12 levels in patients with ischemic stroke
Duan XX, et al.
Neurological Sciences, 36, 2227-2234 (2015)
Discovery of Small Molecule CXCR4 Antagonists
Zhan W, et al.
Journal of Medicinal Chemistry, 50, 5655-5664 (2007)

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