S1629
Sulfatase from Aerobacter aerogenes
Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL
Synonym(s):
Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase
Sign In to View Organizational & Contract Pricing.
Select a Size
10 UNITS
$137.00
50 UNITS
$444.00
$137.00
Check Cart for Availability
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
2-5 units/mg protein (biuret)
Concentration:
10-20 units/mL
Skip To
type
Type VI
form
buffered aqueous glycerol solution
specific activity
2-5 units/mg protein (biuret)
mol wt
~41 kDa
concentration
10-20 units/mL
foreign activity
β-Glucuronidase ≤10 U/mL
shipped in
wet ice
storage temp.
−20°C
Quality Level
Looking for similar products? Visit Product Comparison Guide
Related Categories
1 of 4
This Item | S9751 | S9626 | S9754 |
|---|---|---|---|
| specific activity 2-5 units/mg protein (biuret) | specific activity - | specific activity - | specific activity 20-40 units/mg solid |
| concentration 10-20 units/mL | concentration ≥2,000 units/mL | concentration - | concentration - |
| form buffered aqueous glycerol solution | form aqueous solution | form powder | form lyophilized powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
| mol wt ~41 kDa | mol wt ~85 kDa by gel filtration | mol wt ~85 kDa by gel filtration | mol wt ~43 kDa by gel filtration |
General description
Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.[1]
Application
Sulfatase from Aerobacter aerogenes has been used:
- as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses[2]
- in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1[1]
- to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides[3]
Biochem/physiol Actions
Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging[4] and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis.[1] Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates.[5] Sulfatases find application in industry and agriculture.[1]
Physical form
Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 °C.
used together
Product No.
Description
Pricing
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Toshiyuki Nakamura et al.
Bioscience, biotechnology, and biochemistry, 75(8), 1506-1510 (2011-08-09)
β-Glucuronidase and sulfatase are the major deconjugating enzymes used in the cleavage of the glucuronate and sulfate moieties, respectively, from certain conjugated food factors including polyphenols. In the present study, we found that compounds having the same molecular weights as
Melanie Glauser et al.
Clinica chimica acta; international journal of clinical chemistry, 430, 125-128 (2014-01-15)
Total (i.e. free+sulfated) metanephrines in plasma is a biomarker for the diagnosis of pheochromocytoma/paraganglioma. Sulfated metanephrines must be completely deconjugated by perchloric acid hydrolysis or sulfatase treatment prior to analytical measurement to enable quantification by current techniques. In this report
T Saidha et al.
Archives of biochemistry and biophysics, 272(1), 237-244 (1989-07-01)
Mitochondria that have been purified from cells of light-grown wild-type Euglena gracilis Klebs var. bacillaris Cori or dark-grown mutant W10BSmL and incubated with 35SO4(2-) and ATP accumulate a labeled compound in the surrounding medium. This compound is also labeled when
Bioluminescent probes of sulfatase activity.
Jason S Rush et al.
Chembiochem : a European journal of chemical biology, 11(15), 2096-2099 (2010-09-28)
C Gil et al.
Biochimica et biophysica acta. Biomembranes, 1861(1), 161-169 (2018-11-23)
Epsilon toxin (Etx) from Clostridium perfringens is synthesized as a very low-active prototoxin form (proEtx) that becomes active upon proteolytic activation and has the capacity to cross the blood-brain barrier (BBB), thereby producing severe neurological effects. The identity and requirements
Related Content
Instructions
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service