≥98% (HPLC), solid

6,7-Dimethyl-3-methyl2-methyl1-3-(trifluoromethyl)phenyl-1H-indol-3-ylmethylaminoethylaminomethyl-(4H-1-Benzopyran-4-one dihydrochloride, SPD00000304
Empirical Formula (Hill Notation):
C32H32F3N3O2 · 2HCl
Molecular Weight:
MDL number:
PubChem Substance ID:
Pricing and availability is not currently available.


≥98% (HPLC)






H2O: >5 mg/mL

storage temp.


SMILES string




InChI key


Gene Information

human ... TNF(7124)
mouse ... TNF(21926)
rat ... TNF(24835)


SPD304 has been used in the inhibition of tumor necrosis factor receptor 1 (TNFR1) in dorsal root ganglion neurons and tumor necrosis factor alpha in colonic epithelial cell lines.
SPD304 may be used in TNF-α-mediated cell signaling studies.


1, 5 mg in glass bottle

Biochem/physiol Actions

SPD304 is a small molecule inhibitor of TNF-a activity with a novel mechanism of action. It interferes with protein-protein interactions on the contact surfaces of the trimeric TNF-a subunits, displacing one of the trimer units, resulting in dissociation of the trimer and loss of activity at the receptor TNFR1. SPD304 inhibits TNFR1 receptor binding to TNF-a with an IC50 of 22μM in vitro and inhibits TNF-a-mediated stimulation of IkB degradation in HeLa cells with an IC50 of 4.6 μM.
Trimerization of TNF-α is essential for its biological activity. SPD304 inhibits the trimerization by interacting with Glycine 122 residue. It also inhibits the activity of Receptor activator of nuclear factor-κB ligand (RANKL) by similar interaction.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Hazard Codes


Risk Statement


Safety Statement



NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Molly M He et al.
Science (New York, N.Y.), 310(5750), 1022-1025 (2005-11-15)
We have identified a small-molecule inhibitor of tumor necrosis factor alpha (TNF-alpha) that promotes subunit disassembly of this trimeric cytokine family member. The compound inhibits TNF-alpha activity in biochemical and cell-based assays with median inhibitory concentrations of 22 and 4.6...
Dysregulated Up-Frameshift Protein 1 Promotes Ulcerative Colitis Pathogenesis Through the TNFR1-NF-kappaB/MAPKs Pathway
Zhu H, et al.
Digestive Diseases and Sciences, 63(10), 2593-2603 (2018)
Anthi Mettou et al.
SLAS discovery : advancing life sciences R & D, 23(1), 84-93 (2017-06-07)
The aim of this study is to improve the aqueous solubility of a group of compounds without interfering with their bioassay as well as to create a relevant prediction model. A series of 55 potential small-molecule inhibitors of tumor necrosis...
Cannabinoid WIN-55,212-2 mesylate inhibits tumor necrosis factor-alpha-induced expression of nitric oxide synthase in dorsal root ganglion neurons
Tan R and Cao L
International Journal of Molecular Medicine, 42(2), 919-925 (2018)
Yang Yu et al.
Hypertension (Dallas, Tex. : 1979), 74(1), 63-72 (2019-06-04)
TNF-α (tumor necrosis factor-α) is initially synthesized as a transmembrane protein that is cleaved by TACE (TNF-α-converting enzyme) to release soluble TNF-α. The elevated level of TNF-α in the brain and circulation in heart failure (HF) suggests an increase in...

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