Documents
S1797
SOC Medium
For use in transformation
Quality Level
grade
for molecular biology
sterility
0.2 μm filtered
form
liquid
composition
Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L
Featured Industry
Agriculture
Food and Beverages
storage temp.
2-8°C
suitability
nonselective for Escherichia coli
nonselective for coliforms
Related Categories
General description
Application
Features and Benefits
- Filter-sterilized, ready-to-use format
- Convenient package sizes
- Standard formulation
Preparation Note
2. Autoclave for 15 minutes at 121°C.
RIDADR
NONH for all modes of transport
WGK Germany
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Which bacterial culture medium is the best choice for my application?
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
Why is SOC Medium (S1797) used to recover bacterial cells before transformation?
SOC is a rich bacterial broth that also contains glucose, which aids in the recovery process and prepares the cells for efficient transformation. The use of other media, such as LB - Lennox may also be acceptable, but the recovery and transformation may not be as efficient.
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