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S1797

Sigma-Aldrich

SOC Medium

For use in transformation

Synonym(s):
Super Optimal broth
NACRES:
NA.85

Quality Level

grade

for molecular biology

sterility

0.2 μm filtered

form

liquid

composition

Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

application(s)

agriculture
food and beverages

storage temp.

2-8°C

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

SOC is a microbial growth medium used for the transformation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Application

SOC Medium is a rich media used primarily in the recovery step of Escherichia coli competent cell transformations. Use of SOC maximizes the transformation efficiency of competent cells.

Features and Benefits

SOC liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package sizes
  • Standard formulation

Preparation Note

1. Suspend 31.3 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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