S1797

SOC Medium

For use in transformation

• Synonym(s): Super Optimal broth
• NACRES: NA.85

Properties

• Quality Level: 500
• grade: for molecular biology
• sterility: 0.2 μm filtered
• form: liquid
• composition: Dextrose, 3.603 g/L ; KCl, 0.186 g/L ; MgSO₄, 4.8 g/L ; Tryptone, 20 g/L ; Yeast extract, 5 g/L
• application(s): agriculture; food and beverages
• storage temp.: 2-8°C
• suitability: nonselective for Escherichia coli; nonselective for coliforms

Description

General description

SOC is a microbial growth medium used for the transformation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Application

SOC Medium is a rich media used primarily in the recovery step of Escherichia coli competent cell transformations. Use of SOC maximizes the transformation efficiency of competent cells.

Features and Benefits

SOC liquid provides:

• Filter-sterilized, ready-to-use format
• Convenient package sizes
• Standard formulation

Preparation Note

1. Suspend 31.3 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable