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S1797

Sigma-Aldrich

SOC Medium

For use in transformation

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Synonym(s):
Super Optimal broth
NACRES:
NA.85

grade

for molecular biology

sterility

0.2 μm filtered

form

liquid

composition

Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

application(s)

agriculture

storage temp.

2-8°C

suitability

nonselective for Escherichia coli
nonselective for coliforms

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This Item
1.05470M142885469
Sigma-Aldrich

Sigma-Aldrich

S1797

SOC Medium

SIM medium suitable for microbiology

Millipore

1.05470

SIM medium

MIO Medium suitable for microbiology, NutriSelect® Plus

Millipore

M1428

MIO Medium

Millipore

Millipore

85469

SOC Broth

form

liquid

form

medium granules (dehydrated (DCM))

form

powder

form

powder

application(s)

agriculture

application(s)

food and beverages, microbiology

application(s)

clinical testing
environmental
food and beverages
veterinary, microbiology

application(s)

food and beverages
life science and biopharma, microbiology

storage temp.

2-8°C

storage temp.

15-25°C

storage temp.

-

storage temp.

2-8°C

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

Escherichia coli, coliforms, enterobacteriaceae

suitability

Escherichia coli, nonselective and differential for Enterobacter spp., nonselective and differential for Klebsiella spp., nonselective and differential for Proteus spp., nonselective and differential for enterobacteriaceae

suitability

nonselective for Escherichia coli, nonselective for coliforms

Quality Level

500

Quality Level

200

Quality Level

200

Quality Level

100

General description

Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector. SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Application

SOC Medium has been used for the transformation of DH5α competent E-coli cells. It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest.

Features and Benefits

SOC liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package sizes
  • Standard formulation

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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S Zhao et al.
Applied and environmental microbiology, 75(24), 7624-7630 (2009-10-27)
Ampicillin-resistant (Amp(r)) Salmonella enterica isolates (n = 344) representing 32 serotypes isolated from retail meats from 2002 to 2006 were tested for susceptibility to 21 other antimicrobial agents and screened for the presence of five beta-lactamase gene families (bla(CMY), bla(TEM)
Qiao-Yang Sun et al.
Analytical biochemistry, 394(1), 144-146 (2009-07-23)
In this paper, we report a useful protocol for cloning toxic protein genes. Use of the SOC medium, which is a glucose-containing rich medium, significantly improved the transformation efficiency of a recombinant plasmid containing a toxic plant subtilase SaSBT1 cDNA.
Rebekah M Martin et al.
mSystems, 3(3) (2018-07-03)
Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients
Daniel C Volke et al.
Bio-protocol, 11(4), e3917-e3917 (2021-03-19)
Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-assisted
Detection of Cryptosporidium parvum Oocysts on Fresh Produce Using DNA Aptamers
Iqbal A, et.al.
PLoS ONE (2015)
View All Related Papers

Articles

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Protocols

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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