S1816

Sigma-Aldrich

SYBR® Green JumpStart Taq ReadyMix for Quantitative PCR, Capillary Formulation

SYBR® Green qPCR reagent for Roche LightCycler® capillary systems

NACRES:
NA.55
Pricing and availability is not currently available.

Quality Level

form

liquid

feature

hotstart

concentration

1 units/reaction (20 μL reaction volume)

color

colorless

compatibility

for use with Roche LightCycler 480

shipped in

wet ice

storage temp.

−20°C

Related Categories

Application

For SYBR based capillary qPCR, optimized for use on Roche Lightcycler

Features and Benefits

  • Convenient 2× concentrate ReadyMix specifically designed for use with capillary instruments such as the Roche LightCycler and is ideal for high throughput applications
  • Increased specificity & target yield - JumpStart Taq polymerase prevents non-specific product resulting in more accurate CT values and improved quantitation

Packaging

Default reaction volume is 20 μL

100RXN is packaged as 1 X 1 mL
400RXN is packaged as 1 X 4 mL
A tube of 25 mM MgCl2 is provided for easy optimization of the QPCR reaction.

Other Notes

SYBR Green JumpStart Taq ReadyMix, Capillary formulation combines the advantages of a hot start enzyme, JumpStart Taq, in a 2× concentrate ReadyMix specifically designed for use with capillary instruments, such as the Roche LightCycler® real-time thermal cycler. SYBR Green JumpStart Taq ReadyMix is an optimized formulation containing SYBR Green I dye, JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer and stabilizers.

SYBR Green Taq ReadyMix is recommended for single product real-time amplification experiments and may also be used for evaluation of primer sequences prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye.

Principle

SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. Specificity of Sigma′s SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.

The JumpStart Taq antibody inactivates the DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate CT values.

To prepare a reaction, 10 μL of ReadyMix is added to primers, template and water for a final reaction volume of 20 μL.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
LightCycler is a registered trademark of Roche
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, Third Edition (2000)
Aifeng Liu et al.
Journal of nanoscience and nanotechnology, 19(1), 91-97 (2018-10-18)
Osteoarthritis (OA) is an unavoidable degenerative disease of the human body. A relatively efficient and desirable treatment exists that leads to the ecological restoration of cartilage through the adjustments of the micro-environment of the human body and relies on its...
S A Bustin
Journal of molecular endocrinology, 29(1), 23-39 (2002-08-30)
The fluorescence-based real-time reverse transcription PCR (RT-PCR) is widely used for the quantification of steady-state mRNA levels and is a critical tool for basic research, molecular medicine and biotechnology. Assays are easy to perform, capable of high throughput, and can...
T B Morrison et al.
BioTechniques, 24(6), 954-958 (1998-06-19)
Continuous fluorescence observation of amplifying DNA allows rapid and accurate quantification of initial transcript copy number. A simple and generic method for monitoring product synthesis with the double-stranded DNA dye, SYBR Green I provides initial template copy number estimation limited...
Validation of Quantitative PCR Assays
Lovatt, A., et al.
BioPharm., March, 22-23 (2002)
Articles
Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer
Read More
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
Read More
Protocols
A protocol that can be used as a basic template for qPCR incorporating SYBR Green I DNA binding dye that is amenable to modification and applicable for use as validation for a set of primers.
Read More
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
Read More
Related Content
SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR
Read More
RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.