S1816

SYBR^® Green JumpStart^™ Taq ReadyMix^™ for Quantitative PCR, Capillary Formulation

SYBR^® Green qPCR reagent for Roche LightCycler^® capillary systems

• NACRES: NA.55

Properties

• form: liquid
• usage: sufficient for 100 reactions; sufficient for 400 reactions
• feature: dNTPs included; hotstart
• concentration: 1 units/reaction (20 μL reaction volume)
• technique(s): qPCR: suitable
• color: colorless
• input: purified DNA
• compatibility: for use with Roche LightCycler 480
• detection method: SYBR^® Green
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

SYBR^® Green JumpStart^™ Taq ReadyMix^™, Capillary formulation combines the advantages of a hot start enzyme, JumpStart Taq, in a 2× concentrate ReadyMix specifically designed for use with capillary instruments, such as the Roche LightCycler^® real-time thermal cycler. SYBR Green JumpStart Taq ReadyMix is an optimized formulation containing SYBR Green I dye, JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer and stabilizers.

SYBR Green Taq ReadyMix is recommended for single product real-time amplification experiments and may also be used for evaluation of primer sequences prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye.

Application

SYBR^® Green JumpStart^™ Taq ReadyMix^™ for Quantitative PCR, Capillary Formulation has been used in a real-time quantitative polymerase chain reaction (RT-qPCR) and reverse transcription PCR (RT-PCR).

Features and Benefits

• Convenient 2× concentrate ReadyMix specifically designed for use with capillary instruments such as the Roche LightCycler^® and is ideal for high throughput applications
• Increased specificity & target yield - JumpStart^™ Taq polymerase prevents non-specific product resulting in more accurate C_T values and improved quantitation
• This master mix allows consistency from one reaction to the next
• Designed to reduce the preparation time and minimize contamination from multiple pipetting steps
• The double-strand DNA-specific SYBR^® Green I fluorescent dye is inexpensive, easy to use, and sensitive and is ideal for quantifying any DNA sequence

Packaging

A tube of 25 mM MgCl₂ is provided for easy optimization of the QPCR reaction.

Default reaction volume is 20 μL

100RXN is packaged as 1 X 1 mL
400RXN is packaged as 1 X 4 mL

Principle

SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. Specificity of Sigma′s SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.

The JumpStart Taq antibody inactivates the DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate C_T values.

To prepare a reaction, 10 μL of ReadyMix is added to primers, template and water for a final reaction volume of 20 μL.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

JumpStart is a trademark of Sigma-Aldrich Co. LLC

LightCycler is a registered trademark of Roche

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable