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S2014

Sigma-Aldrich

Staphylococcus aureus

buffered aqueous suspension, Wood 46 strain

NACRES:
NA.81

biological source

Staphylococcus aureus

Quality Level

sterility

Not processed or packaged aseptically

form

buffered aqueous suspension

composition

Cell suspension, ~10% wet weight/volume

storage temp.

2-8°C

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P6031P7155539202
Sigma-Aldrich

Sigma-Aldrich

S2014

Staphylococcus aureus

biological source

Staphylococcus aureus

biological source

Staphylococcus aureus

biological source

-

biological source

-

form

buffered aqueous suspension

form

essentially salt-free, lyophilized powder

form

cell suspension

form

lyophilized solid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

sterility

Not processed or packaged aseptically

sterility

-

sterility

(not processed or packaged aseptically)

sterility

-

composition

Cell suspension, ~10% wet weight/volume

composition

-

composition

-

composition

-

General description

Staphylococcus aureus Wood 46 is protein A deficient and spa negative. It shares 98% to 99% genome identity with S.aureus and shows a lower surface expression of cell wall-associated protein A.

Application

(Not intended for use as a starter culture.)
Staphylococcus aureus has been used:
  • to mimic infection and induce fever in Pekin duck
  • to test its effect on hemocyte morphology in hemolymph samples from beetle Tenebrio molitor larva
  • in the antibacterial activity and minimum inhibitory concentration (MIC) assay with gedunin and 7-deacetoxy-7αhydroxygedunin potassium salt

Wood 46, a non-protein A producing S. aureus strain, prepared by the same method as P7155 (Protein A, crude cell suspension-Cowan strain), may be used as a control in protein A-immunoglobulin binding studies.

Biochem/physiol Actions

Staphylococcus aureus Wood 46 displays reduced virulence compared to the S. aureus. This isolate is useful in understanding protein A role in pathogenesis and virulence.

Physical form

Formalin-fixed crude cell suspension of essentially non-viable S. aureus (Wood 46 strain) in 0.05 M potassium phosphate buffer, pH 7.5, containing 0.2% sodium azide

Preparation Note

Produced in pure culture.

Analysis Note

This strain binds less than 10% of the rabbit IgG bound by P 7155 as assayed by a modification of the method of Kessler.

Storage Class Code

6.2 - Infectious substances

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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