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S2147

Sigma-Aldrich

Monoclonal Anti-Superoxide Dismutase (SOD) antibody produced in mouse

clone SD-G6, ascites fluid

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Synonym(s):
Anti-ALS, Anti-ALS1, Anti-HEL-S-44, Anti-IPOA, Anti-SOD, Anti-STAHP, Anti-hSod1, Anti-homodimer
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SD-G6, monoclonal

contains

15 mM sodium azide

species reactivity

canine, human, rat

technique(s)

indirect ELISA: 1:300

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SOD1(6647)
rat ... Sod1(24786)

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This Item
S5069SAB4200807SAB2500976
conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

antibody form

ascites fluid

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

clone

SD-G6, monoclonal

clone

polyclonal

clone

SD-G6, monoclonal

clone

polyclonal

species reactivity

canine, human, rat

species reactivity

rat, human

species reactivity

human, rat, canine

species reactivity

rat, human, canine, mouse

technique(s)

indirect ELISA: 1:300

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using biotin/ExtraAvidin®-peroxidase staining of human heart sections, indirect immunofluorescence: 10-20 μg/mL using methanol-acetone-fixed rat NRK cells, western blot: 1-2 μg/mL using human HeLa mitochondria extract and a chemiluminescent detection reagent, western blot: 2-4 μg/mL using whole extract of rat brain and a chemiluminescent detection reagent

technique(s)

indirect ELISA: 0.3-0.6 μg/mL using 5μg/ml Superoxide Dismutase from human erythrocytes for coating

technique(s)

indirect ELISA: suitable, western blot: suitable

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General description

Monoclonal Anti-Superoxide Dismutase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Superoxide Dismutase (SOD) is a family of metalloenzymes widely distributed in both plants and animals. In mammalian tissues, three types of superoxide dismutase [Cu-Zn-SOD, Mn-SOD, extracellular (EC)-SOD] occur. Human manganese superoxide dismutase (MnSOD), isolated from liver is composed of 22 kDa subunits each containing one Mn atom, while SOD from bovine erythrocyte has a molecular weight of 32.5 kDa. Superoxide Dismutase occurs in high concentrations in brain, liver, heart, erythrocytes and kidney.
Superoxide Dismutase (SOD) or CuZn-SOD (SOD1), a cytoplasmic and mitochondrial intermembrane space protein is located on human chromosome 21q22. It belongs to superoxide dismutase multigene family.

Specificity

The antibody recognizes natural and recombinant human-Cu-Zn-SOD, human placental SOD, and human erythrocyte SOD using direct capture or competitive ELISA. Cross-reactivity has been observed with human liver and salivary gland, rat salivary gland, pheochromocytoma cell line (PC12), and dog salivary gland. No reactivity was observed with SOD from bovine erythrocytes, kidney, and liver; dog erythrocytes; Bacillus stearothermophilus; E. coli, or horseradish.

Immunogen

recombinant human copper-zinc superoxide dismutase (Cu-Zn-SOD).

Application

Anti-Superoxide Dismutase (SOD) antibody has been used in immunohistochemistry and Cu-Zn SOD detection via ELISA.

Biochem/physiol Actions

Superoxide Dismutase (SOD) appear to protect cells against reactive free radicals by scavenging the superoxide radicals produced by ionization radiation or through other mechanisms. SOD have been proposed as clinically useful for a wide variety of applications including prevention of oncogenesis, tumor promotion, tumor invasiveness, radiation damage, reduction of the cytotoxic and cardiotoxic effects of anticancer drugs, as a measure against the aging process and as anti-inflammatory agents.
Superoxide Dismutase (SOD) or CuZn-SOD (SOD1) mutations results in amyotrophic lateral sclerosis It acts as a mediator of the HMF (hypomagnetic field) effect.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Superoxide Dismutases: Role in Redox Signaling, Vascular Function, and Diseases
Fukai T, et al.
Antioxidants & Redox Signaling, 15(6), 1583-1583 (2011)
Targeted expression of human CuZn superoxide dismutase gene in mouse central nervous system
Li Y, et al.
Journal of Neuroscience Methods, 89(1), 49-55 (1999)
Formation of high molecular weight complexes of mutant Cu, Zn-superoxide dismutase in a mouse model for familial amyotrophic lateral sclerosis
Johnston JA, et al.
Proceedings of the National Academy of Sciences of the USA, 97(23), 12571-12576 (2000)
Marcel Maier et al.
Science translational medicine, 10(470) (2018-12-07)
Mutations in the gene encoding superoxide dismutase 1 (SOD1) lead to misfolding and aggregation of SOD1 and cause familial amyotrophic lateral sclerosis (FALS). However, the implications of wild-type SOD1 misfolding in sporadic forms of ALS (SALS) remain unclear. By screening
J Roy et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 18(23), 9673-9684 (1998-11-21)
Mutations in the Cu/Zn-superoxide dismutase (SOD-1) gene are responsible for a subset of familial cases of amyotrophic lateral sclerosis. Using a primary culture model, we have demonstrated that normally nontoxic glutamatergic input, particularly via calcium-permeable AMPA/kainate receptors, is a major

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