All Photos(3)
Documents
S3194
Stemline® Neural Stem Cell Expansion Medium
without growth factors and antibiotics, liquid, sterile-filtered, suitable for cell culture
All Photos(3)
Synonym(s):
NSC Medium
NACRES:
NA.81
Pricing and availability is not currently available.
Quality Level
sterility
sterile-filtered
form
liquid
drug control
regulated under CDSA - not available from Sigma-Aldrich Canada
application(s)
cell culture | mammalian: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
Stemline® Neural Stem Cell Expansion Medium is an animal component-free formulation optimized specifically for the expansion of human neural stem cells, which retain their differentiative capacity. Stemline Neural Stem Cell Expansion Medium outperforms standard serum-free media formulations for the generation of neural stem cells, without the use of any animal-derived components or the need for additional supplements, other than growth factors.
Application
Stemline® Neural Stem Cell Expansion Medium has been used to expand human neural progenitor cells (hNPCs), human embryonic stem (hES) and induced pluripotent stem (iPS) cells in to free floating spheres.
Components
Stemline® Neural Stem Cell Expansion Medium is a proprietary formulation, which does not contain growth factors, antibiotics, or animal-derived components.
Legal Information
Stemline is a registered trademark of Sigma-Aldrich Co. LLC
RIDADR
NONH for all modes of transport
WGK Germany
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificate of Analysis
Certificate of Origin
Derivation of Myogenic Progenitors Directly From Human Pluripotent Stem Cells Using a Sphere?Based Culture.
Hosoyama T, et al.
Stem Cells Translational Medicine, 3(5), 564-574 (2014)
Aslam Abbasi Akhtar et al.
Stem cell reports, 4(3), 323-331 (2015-02-24)
Precise methods for transgene regulation are important to study signaling pathways and cell lineages in biological systems where gene function is often recycled within and across lineages. We engineered a genetic toolset for flexible transgene regulation in these diverse cellular...
Fusako Sakai-Takemura et al.
Communications biology, 3(1), 182-182 (2020-04-22)
Understanding the signaling pathways that regulate proliferation and differentiation of muscle progenitors is essential for successful cell transplantation for treatment of Duchenne muscular dystrophy. Here, we report that a γ-secretase inhibitor, DAPT (N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenylglycine tertial butyl ester), which inhibits the release...
Eileen Lynch et al.
Disease models & mechanisms, 12(8) (2019-08-24)
Amyotrophic lateral sclerosis (ALS) is a late-onset neuromuscular disease with no cure and limited treatment options. Patients experience a gradual paralysis leading to death from respiratory complications on average only 2-5 years after diagnosis. There is increasing evidence that skeletal muscle...
Fusako Sakai-Takemura et al.
Scientific reports, 8(1), 6555-6555 (2018-04-28)
Human induced pluripotent stem cells (hiPSCs) are a potential source for cell therapy of Duchenne muscular dystrophy. To reliably obtain skeletal muscle progenitors from hiPSCs, we treated hiPS cells with a Wnt activator, CHIR-99021 and a BMP receptor inhibitor, LDN-193189...
Protocols
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
Read MoreRelated Content
Introducing a new cell therapy demands optimal performance in upstream and downstream processing, process development, testing, and manufacturing. World-class products and partners are critical to success, speeding advancement of therapeutics that are effective, safe, and affordable.
Read MoreOur team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical ServiceSupport
Customer SupportContact UsFAQSafety Data Sheets (SDS)Certificates (COA/COO)Quality & RegulatoryCalculators & AppsWebinarsSocial Media
MilliporeSigma
Research. Development. Production.
We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.
© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.
Reproduction of any materials from the site is strictly forbidden without permission.