S3396

Sigma-Aldrich

Monoclonal Anti-Spectrin (α and β) antibody produced in mouse

clone SB-SP1, ascites fluid

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SB-SP1, monoclonal

contains

15 mM sodium azide

species reactivity

human

application(s)

indirect immunofluorescence: suitable using erythrocyte smears
microarray: suitable
western blot: 1:500 using extract of human erythrocyte ghosts or purified human erythrocyte spectrin

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... SPTA1(6708), SPTB(6710)

General description

Spectrins are heterotetramers, that are composed of several αand β subunit isoforms. They are the constituents of G-protein-coupled receptor (GPCR) and the synaptic multiprotein complex. α-Spectrin has 20 spectrin repeats (α1-α20) and β-spectrin has 17 spectrin repeats (β1-β17). SPTA1 (spectrin α 1) is located on chromosome 1q21–q23 and SPTB (spectrin β) is located on chromosome 14q22–q23.2.
Monoclonal anti-Spectrin (α and β) (mouse IgG1 isotype) is produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Spectrin is a major constituent of the erythrocyte skeleton accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass.

Specificity

This antibody reacts specifically with the α and β chains of human erythrocyte spectrin.

Immunogen

human erythrocyte spectrin.

Application

Anti-Spectrin (α and β) antibody has been used in western blotting and immunostaining.
Monoclonal Anti-Spectrin (α and β) antibody produced in mouse has been used in flow cytometry analysis and confocal microscopy.

Biochem/physiol Actions

Spectrins are multifunctional proteins, that modulates the cell morphology and mechanical properties. Spectrins also participates in the cell cycle by controlling the upregulation of membrane receptors. α- and β-spectrins are essential for the development of nervous system.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Kurt L Barkalow et al.
The Journal of cell biology, 161(3), 557-570 (2003-05-14)
Aspectrin-based skeleton uniformly underlies and supports the plasma membrane of the resting platelet, but remodels and centralizes in the activated platelet. alpha-Adducin, a phosphoprotein that forms a ternary complex with F-actin and spectrin, is dephosphorylated and mostly bound to spectrin...
Jan Hirsch et al.
Clinical chemistry, 49(5), 792-799 (2003-04-24)
Localized overheating of packed red blood cells (PRBCs) after microwave warming with consequent damage to erythrocytes has been reported. We therefore compared possible cellular markers of erythrocyte damage, as measured by flow cytometry, with laboratory indicators of hemolysis to evaluate...
Membrane protein carbonylation in non-leukodepleted CPDA-preserved red blood cells
Kriebardis AG, et al.
Blood Cells, Molecules and Diseases, 36(2), 279-282 (2006)
Specificity of commercial anti-spectrin antibody in the study of fungal and Oomycete spectrin: cross-reaction with proteins other than spectrin
Cotado-Sampayo M, et al.
Fungal genetics and biology : FG & B, 45(6), 1008-1015 (2008)
Indicators of erythrocyte damage after microwave warming of packed red blood cells
Hirsch J, et al.
Clinical Chemistry, 49(5), 792-799 (2003)

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