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S3401

Sigma-Aldrich

Sample Buffer, Laemmli 2× Concentrate

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Synonym(s):
SDS Sample Buffer
NACRES:
NA.25

packaging

vial of 6 mL

storage temp.

−20°C

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This Item
S6639T116570607
vibrant-m

S6639

SSC Buffer 20× Concentrate

vibrant-m

70607

4X SDS Sample Buffer

storage temp.

−20°C

storage temp.

room temp

storage temp.

2-8°C

storage temp.

−20°C

packaging

vial of 6 mL

packaging

-

packaging

-

packaging

-

Application

Sample Buffer, Laemmli 2× concentrate has been used as a sample buffer for denaturing and loading of protein samples in SDS-PAGE.

Biochem/physiol Actions

Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well.

Other Notes

Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8.

Quantity

Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.

related product

Product No.
Description
Pricing

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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Protocols

To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.

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