All Photos(3)

S3504

Sigma-Aldrich

Sigmacell Cellulose

Type 20, 20 μm

Synonym(s):
Cotton linters, Cellulose powder, Cellulose
CAS Number:
EC Number:
MDL number:

Quality Level

type

Type 20

particle size

20 μm

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Application

Sigmacell cellulose is used in thin layer chromatography (TLC) and cellulose TLC adsorbents. Sigmacell cellulose has been used in studies to inform industrial applications for large-scale purification of enzymes as well as to investigate the cellulase system of Penicillium echinulatum, which has been identified as a potential cellulase producer for bioconversion processes.
Makes extremely durable layers. Sharp separations for amino acids, sugars and other hydrophilic substances. Development time as short as 2 hours for 8′′ plates.
High purity cellulose powders for partition chromatography.

Packaging

500 g in poly bottle
1 kg in poly bottle

Reconstitution

For TLC, simply blend a 15-20% aqueous slurry for about one minute and coat plates; dry at room temperature. Usually does not require activation before use.

Legal Information

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Stuart M Linton et al.
The Journal of experimental biology, 207(Pt 23), 4095-4104 (2004-10-23)
Digestive juice from the herbivorous gecarcinid land crabs Gecarcoidea natalis and Discoplax hirtipes exhibited total cellulase activity and activities of two cellulase enzymes; endo-beta-1,4-glucanase and beta-1,4-glucosidase. These enzymes hydrolysed native cellulose to glucose. The digestive juice of both species also...
Nasrullah Shah et al.
Carbohydrate polymers, 98(2), 1585-1598 (2013-09-24)
Bacterial cellulose (BC) has received substantial interest owing to its unique structural features and impressive physico-mechanical properties. BC has a variety of applications in biomedical fields, including use as biomaterial for artificial skin, artificial blood vessels, vascular grafts, scaffolds for...
Nathalie Lavoine et al.
Carbohydrate polymers, 90(2), 735-764 (2012-07-31)
Interest in microfibrillated cellulose (MFC) has been increasing exponentially. During the last decade, this bio-based nanomaterial was essentially used in nanocomposites for its reinforcement property. Its nano-scale dimensions and its ability to form a strong entangled nanoporous network, however, have...
Okako Omadjela et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(44), 17856-17861 (2013-10-16)
Cellulose is a linear extracellular polysaccharide. It is synthesized by membrane-embedded glycosyltransferases that processively polymerize UDP-activated glucose. Polymer synthesis is coupled to membrane translocation through a channel formed by the cellulose synthase. Although eukaryotic cellulose synthases function in macromolecular complexes...
Rachel A Burton et al.
Current opinion in biotechnology, 26, 79-84 (2014-04-01)
Plant cell walls consist largely of cellulose, non-cellulosic polysaccharides and lignin. Concerted attempts are underway to convert wall polysaccharides from crop plant residues into renewable transport fuels and other valuable products, and to exploit the dietary benefits of cereal grain...

Protocols

Enzymatic Assay of Cellulase

To standardize an enzymatic assay procedure of cellulase.

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