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S4072

Sigma-Aldrich

Anti-Sirt3 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-SIR2L3, Anti-Sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~28 kDa

species reactivity

human

technique(s)

indirect immunofluorescence: suitable
western blot: 2.5-5 μg/mL using human HepG2 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... SIRT3(23410)

General description

Sirtuin 3 (Sirt3) is an NAD-dependent deacetylase localized to the mitochondrial matrix. SIRT3 encodes a 44 kDa full length form and a N-terminal 142 amino acids truncated form. It is mapped to human chromosome 11p15.5.

Immunogen

synthetic peptide corresponding to amino acids 384-399 of human SIRT3, conjugated to KLH via an N-terminal cysteine residue.

Application

Anti-Sirt3 antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

Sirtuin 3 (Sirt3) is mainly a nuclear protein that transfers to the mitochondria during cellular stress. In humans, Sirt3 deacetylates and activates the mitochondrial enzyme acetyl-CoA synthetase 1 (AceCS2). Sirt3 is implicated in regulating mitochondrial function and thermogenesis in brown adipocytes. Its levels have been shown to correlate with extended life span. Increased levels of Sirt3 were associated with node positive breast cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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