S4320

SYBR^® Green Extract-N-Amp^™ PCR ReadyMix^™

Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

• Synonym(s): SYBR Green PCR Mix
• NACRES: NA.31
• UNSPSC Code: 41106303

Properties

• usage: sufficient for 100 reactions, 1.2 mL sufficient for 100 amplifications, sufficient for 1000 reactions, 12 mL sufficient for 1000 amplifications, sufficient for 10000 reactions, 125 mL sufficient for 10000 amplifications
• feature: dNTPs included, hotstart
• Quality Level: 200
• technique(s): RT-qPCR: suitable, qPCR: suitable
• input: crude DNA
• detection method: SYBR^® Green
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

The SYBR^® Green Extract-N-Amp^™ PCR ReadyMix is the specially formulated PCR enzyme blend contained in the SYBR Green Extract-N-Amp Plant PCR kit and SYBR Green Extract-N-Amp Tissue PCR Kit. It is intended to supplement these kits for researchers who perform more than one amplification per extracted sample.

Application

SYBR^® Green Extract-N-Amp^™ PCR ReadyMix^™ or SyBr Green 2× Master Mix kit has been used in a real-time quantitative polymerase chain reaction (qRT–PCR).[1][2]

Packaging

1.2 mL is sufficient for 100 amplifications as described in kit protocols (20 μl total reaction volume)

12 mL is sufficient for 1000 amplifications as described in kit protocols (20 μl total reaction volume)

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries