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S4760

Sigma-Aldrich

N-Succinyl-Ala-Ala-Ala-p-nitroanilide

elastase substrate

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Synonym(s):
N-Succinyl-L-alanyl-L-alanyl-L-alanine 4-nitroanilide, N-Succinyl-tri-L-alanine 4-nitroanilide
Empirical Formula (Hill Notation):
C19H25N5O8
CAS Number:
Molecular Weight:
451.43
Beilstein:
2928351
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Assay

≥98% (HPLC)

form

powder

solubility

DMF: 25 mg/mL, clear, colorless to light yellow

storage temp.

2-8°C

SMILES string

C[C@H](NC(=O)CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)Nc1ccc(cc1)[N+]([O-])=O

InChI

1S/C19H25N5O8/c1-10(20-15(25)8-9-16(26)27)17(28)21-11(2)18(29)22-12(3)19(30)23-13-4-6-14(7-5-13)24(31)32/h4-7,10-12H,8-9H2,1-3H3,(H,20,25)(H,21,28)(H,22,29)(H,23,30)(H,26,27)/t10-,11-,12-/m0/s1

Inchi Key

GVUGADOWXGKRAE-SRVKXCTJSA-N

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This Item
S7632M4765B7632
form

powder

form

powder

form

powder

form

powder

solubility

DMF: 25 mg/mL, clear, colorless to light yellow

solubility

DMF: 10 mg/mL, clear, colorless

solubility

DMF: soluble 25 mg/mL, clear, colorless to light yellow

solubility

methanol: 20 mg/mL, clear, colorless to light yellow

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

General description

N-Succinyl-Ala-Ala-Ala-p-nitroanilide is a chromogenic substrate for chymotrypsin-like serine protease, pancreatic elastase and serine endopeptidase.

Application

N-Succinyl-Ala-Ala-Ala-p-nitroanilide has been used as a substrate
  • for screening elastase activity in lyophilized earthworm fibrinolytic enzymes
  • for quantifying elastase activity in sheep milk
  • in screening honeybee inhibitors in serine protease inhibition assay

Substrates

Elastase substrate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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T Fera et al.
The American review of respiratory disease, 133(4), 568-573 (1986-04-01)
In order to determine the acute effect of smoking on elastase in bronchoalveolar lavage fluid (BAL), we obtained BAL from 30 smokers twice, the first before smoking (after 8 h of abstinence) and the second 2 min to 1 h
Vinu S Siva et al.
Fish & shellfish immunology, 59, 1-8 (2016-11-05)
Serine protease inhibitors (SPIs) play a crucial role in regulation of both host and bacterial serine protease. They are classified into several protein families, where Kazal-type inhibitors are one of families with multi-domain. In the present study, the polymorphism of
Hu Wan et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 165(1), 36-41 (2013-03-19)
Spider-derived Kunitz-type serine protease inhibitors have been shown to exhibit plasmin and elastase inhibition activity and potassium channel blocking activity, but thus far, no additional roles for spider-derived chymotrypsin inhibitors have been elucidated. In this study, a spider (Araneus ventricosus)
M Ogawa et al.
Research communications in chemical pathology and pharmacology, 55(2), 271-274 (1987-02-01)
A human urinary trypsin inhibitor preparation, MR-20, strongly inhibited granulocyte elastase activity. The concentration of MR-20 causing 50% inhibition of the hydrolysis of Suc(Ala) 3pNA by granulocyte elastase was estimated to be 6.4 X 10(-8) M (11 U/ml).
William R Swindell et al.
International journal of molecular sciences, 22(16) (2021-08-28)
Tetrahexyldecyl Ascorbate (THDC) is an L-ascorbic acid precursor with improved stability and ability to penetrate the epidermis. The stability and transdermal penetration of THDC, however, may be compromised by the oxidant-rich environment of human skin. In this study, we show

Articles

Elastase

Enzyme Explorer Product Application Index for Elastase. Leukocyte elastase is a 29KDa serine endoprotease of the Proteinase S1 Family. It exists as a single 238 amino acid-peptide chain with four disulfide bonds.

Protocols

Enzymatic Assay of Elastase (EC 3.4.21.36)

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

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