S4920
SITE Liquid Media Supplement (100×)
liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonym(s):
Media supplement
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 5 mL | Check Cart for Availability | $57.00 |
About This Item
NACRES:
NA.75
UNSPSC Code:
12352205
sterility
sterile-filtered
Quality Level
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
shipped in
ambient
storage temp.
2-8°C
General description
SITE Liquid Media Supplement (100×) serves as a substitute for serum-free formulations. It contains purified factors required for in vitro growth and differentiation studies. The addition of supplements to media will vary, depending on the cell type being studied and the basal medium employed. It is a general cell supplement designed for use in non-complex media (e.g., minimum essential medium (MEM), Roswell Park Memorial Institute Medium (RPMI-1640)) and complex media (e.g., Ham′s F-12, Dulbecco′s Modified Eagle Medium (DME) /F-12, MEM) with sodium pyruvate.
Application
Biochem/physiol Actions
- Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.[4]
- Transferrin: an iron-transport protein. Iron is an essential trace element but can be toxic in its free form. To nourish cells in culture, iron is supplied bound to transferrin in serum.[5]
- Selenium: an essential trace element normally provided by serum.[6]
- Ethanolamine: plays a significant role in the proliferation of hybridoma cells and frequently is added to supplements used for culturing these cells.[7]
Preparation Note
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
Other Notes
Contains 1.0mg/ml recombinant human insulin, 0.55mg/ml human transferrin (substantially iron-free), 0.5μg/ml sodium selenite and 0.2mg/ml ethanolamine at the 100x concentration.
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This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form liquid | form liquid | form liquid |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| Quality Level 500 | Quality Level 500 | Quality Level 500 | Quality Level 300 |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
Lixin Zhu et al.
American journal of physiology. Cell physiology, 295(1), C192-C202 (2008-05-16)
In a comparison of three different tissues, the membrane cytoskeleton linker protein ezrin was found to assume high levels of phosphorylation on threonine-567 (T567) in the brush border membranes of renal proximal tubule cells and small intestine enterocytes, in contrast
H Murakami et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(4), 1158-1162 (1982-02-01)
A serum-free medium supplemented with a few growth factors was devised to grow lymphocyte hybridomas. The medium was developed with the hybridoma line MPC11-BL, a fusion product between a mouse plasmacytoma cell line (MPC11TG70na3) and mouse (BALB/c) spleen cells. In
Rawan Eid et al.
Biochimica et biophysica acta. Molecular cell research, 1864(2), 399-430 (2016-12-13)
Iron is an essential micronutrient that is problematic for biological systems since it is toxic as it generates free radicals by interconverting between ferrous (Fe2+) and ferric (Fe3+) forms. Additionally, even though iron is abundant, it is largely insoluble so
Global Trade Item Number
| SKU | GTIN |
|---|---|
| S4920-5ML | 04061838260017 |