Yes, SYPRO Ruby Protein Blot Stain works with both nitrocellulose and polyvinylidene difluoride (PVDF) membranes. For more information, please access the Product Data Sheet which can be found in the DOCUMENTATION section under the 'More Documents' tab:
https://www.sigmaaldrich.com/product/sigma/s4942#product-documentation
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/369/103/s4817dat.pdf
S4942
SYPRO™ Ruby Protein Gel Stain
Synonym(s):
protein gel stain, SYPRO™ dye
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 200 mL | Available to ship TODAYfromMILWAUKEE | $191.00 | |
| 1 L | Check Cart for Availability | $651.00 | |
| 5 L | Available to ship TODAYfromMILWAUKEE | $2,640.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
41105322
$191.00
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shelf life
≥6 mo. (when stored at room temperature and protected from light)
Quality Level
technique(s)
protein staining: suitable
fluorescence
λex 280,450 nm; λem 610 nm
General description
SYPRO Ruby protein gel stain is a ready-to-use, ultrasensitive, luminescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). This stain, designed especially for use in 2-D PAGE, has proven to be an excellent choice for 1-D PAGE and isoelectric focusing (IEF) gels as well. SYPRO Ruby protein gel stain attains sensitivity comparable to many silver stain techniques. However, unlike silver staining, the SYPRO Ruby gel stain:
- uses a simple staining protocol, with no possibility of overstaining
- delivers a linear quantitation range of over three orders of magnitude
- shows less protein-to-protein variability
- stains glycoproteins, lipoproteins, calcium-binding proteins, fibrillar proteins, and other difficult-to-stain proteins
- will not stain extraneous nucleic acids
- does not interfere with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry
Application
SYPRO ruby protein gel stain has been used for staining of the proteins after sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE).[1]
Legal Information
SYPRO is a trademark of Thermo-Fisher Scientific, Inc.
Disclaimer
Protect from light.
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This Item | |||
|---|---|---|---|
| technique(s) protein staining: suitable | technique(s) protein staining: suitable | technique(s) protein staining: suitable | technique(s) PCR: suitable |
| fluorescence λex 280,450 nm; λem 610 nm | fluorescence λex 300,470 nm; λem 570 nm | fluorescence - | fluorescence - |
| shelf life ≥6 mo. (when stored at room temperature and protected from light) | shelf life ≥6 mo. (when stored desiccated and protected from light at room temperature, 4 °C or 20 °C.) | shelf life - | shelf life - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Protocols
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
Articles
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| S4942-200ML | 04061838256362 |
| S4942-5L | 04061836933746 |
| S4942-1L | 04061838256355 |
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Salve Sybro ruby è utilizzabile per colorare membrane blot PVDF?
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