MilliporeSigma
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S5059

Sigma-Aldrich

SigmaSpin Sequencing Reaction Clean-Up

post-reaction clean-up columns

Synonym(s):
sequencing clean up columns
NACRES:
NA.52

Quality Level

storage temp.

2-8°C

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Application

SigmaSpin® Sequencing Reaction Clean-Up has been used in the synthesis of antisense RNA probe.
Ideal for removing dye-terminator nucleotides and primers from sequencing reactions and radiolabeled nucleotides, primers, and fluorescent dyes from nucleic acid probe labeling reactions.

Other Notes

SigmaSpin Post-Reaction Clean-Up Columns are ideal for lower throughput applications, such as clean-up of probe labeling reactions or small numbers of sequencing reactions. These columns can accept sample volumes up to 100 μL. Each column comes with a collection tube to collect the DNA during centrifugation.

SigmaSpin Post-Reaction Clean-Up columns provide a fast, simple, and highly efficient method for removing unincorporated dyes, excess salts, and other interfering reaction components. Each column is packed with a pre-hydrated size-exclusion resin, equilibrated with molecular biology grade water, and supplied in our unique column design. The column also includes a snap-cap bottom seal and an o-ring containing screw-cap to ensure that the resin remains hydrated. SigmaSpin has been tested in high-throughput genome centers and core facilities with ABI PRISM® 3700, 3100, 310 and 377. Each well can accept sample volumes up to 20 μL.
For additional information, please see www.sigma-aldrich.com/sequencing.

Legal Information

ABI PRISM is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SabreLite is a registered trademark of Pelican Products, Inc.
SigmaSpin is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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