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S5395

Sigma-Aldrich

Superoxide Dismutase from bovine erythrocytes

≥3,000 units/mg protein, BioReagent, lyophilized powder, suitable for cell culture

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Synonym(s):
SOD, Superoxide: superoxide oxidoreductase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.75

biological source

bovine erythrocytes

product line

BioReagent

form

lyophilized powder

specific activity

≥3,000 units/mg protein

mol wt

32.5 kDa

packaging

pkg of 15000 units

technique(s)

cell culture | mammalian: suitable

pH

7.6-10.5

shipped in

dry ice

storage temp.

−20°C

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This Item
S7446574594S7571
biological source

bovine erythrocytes

biological source

-

biological source

bovine erythrocytes

biological source

bovine

specific activity

≥3,000 units/mg protein

specific activity

≥4,500 units/mg protein

specific activity

≥3000 units/mg dry wt.

specific activity

≥3,000 units/mg protein

mol wt

32.5 kDa

mol wt

32.5 kDa

mol wt

-

mol wt

32.5 kDa

pH

7.6-10.5

pH

-

pH

-

pH

-

shipped in

dry ice

shipped in

-

shipped in

ambient

shipped in

-

General description

Superoxide Dismutase from bovine erythrocytes is a metalloprotein which disproportionates superoxide anion radicals. It is a 31.5 kDa copper binding protein and displays a conserved domain and fold. It is a homodimer with one copper and zinc ion per subunit and has antiparallel “greek-key” β barrel fold.

Application

Superoxide Dismutase (SOD) from bovine erythrocytes has been used:
  • for measuring the superoxide radical using the electron paramagnetic resonance spin in human brain microvascular endothelial cells
  • for measuring superoxide production in cytochrome C assay in peripheral blood mononuclear cells
  • as a standard in characterization of hen egg SOD using Fourier-transform infrared spectroscopy (FTIR) and matrix-assisted laser desorption/ionization (MALDI) analysis

Biochem/physiol Actions

Superoxide Dismutase from bovine erythrocytes catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It serves as an antioxidant and plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Unit Definition

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.

Analysis Note

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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