MilliporeSigma
All Photos(1)

Documents

S5447

Sigma-Aldrich

Anti-SIRT1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Sign Into View Organizational & Contract Pricing

Synonym(s):
Anti-S. cerevisiae, HOMOLOG-LIKE 1, Anti-SIR2, Anti-SIR2α, Anti-SIR2L1, Anti-Sirtuin 1
MDL number:
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~120 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using extracts of Jurkat cells
western blot: 2.5-5 μg/mL using whole extracts of Jurkat cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SIRT1(23411)
mouse ... Sirt1(93759)
rat ... Sirt1(309757)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB5700606HPA052351HPA006295
conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

mol wt

antigen ~120 kDa

mol wt

61, 81

mol wt

-

mol wt

-

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Anti-SIRT1 recognizes human SIRT1. Sirtuin 1 (SIRT1) is encoded by the gene mapped to human chromosome 10q21.3. The encoded protein belongs to the silent information regulator (SIR) family and is a class of HDAC (histone deacetylase). SIRT1 is expressed in various tissues including brain, liver, pancreas, adipose tissue, and skeletal muscle. SIRT1 is also known as the longevity gene.

Immunogen

synthetic peptide corresponding to amino acids 580-593 of human SIRT1, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence differs by three amino acids in rat and mouse Sirt1.

Application

Anti-SIRT1 antibody produced in rabbit has been used in immunohistochemistry.

Biochem/physiol Actions

Sirtuin 1 (SIRT1) functions as a regulator of various metabolic pathways, and influences the pathophysiology of several metabolic diseases. It is a regulator of protein deacetylation, and is a candidate therapeutic target in non-alcoholic fatty liver disease (NAFLD), amyotrophic lateral sclerosis (ALS), kidney disease, and pulmonary disease. It also participates in tumorigenesis, and whether it functions as an oncogene or as a tumor suppressor depends upon the tumor type. In pancreatic ductal adenocarcinoma (PDAC), its elevated expression is linked with poor prognosis, and in non-small-cell lung cancer (NSCLC), it suppresses the expression of tumor suppressor p27. The encoded protein inhibits the terminal differentiation in dividing myocytes by binding to the myoD cofactor, pCAF. It is also thought to function as a suppressor of cardiovascular disorders, such as myocardial infarction, or neurodegenerative diseases, such as Alzheimer′s disease (AD) or Parkinson′s disorder (PD).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Other Notes

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Cloning, chromosomal characterization and mapping of the NAD-dependent histone deacetylases gene sirtuin 1.
Voelter-Mahlknecht S and Mahlknecht U
International Journal of Molecular Medicine, 17, 59-67 (2006)
17?-Estradiol Attenuates Hematoma Expansion through ERa/Sirt1/NF-?B Pathway in Hyperglycemic Intracerebral Hemorrhage Mice
Zheng Y, et al.
Stroke, 46, 485?491-485?491 (2015)
Diana J Liu et al.
Journal of cellular physiology, 229(9), 1224-1235 (2014-01-18)
Neurons have a limited capacity for heat shock protein (HSP) induction and are vulnerable to the pathogenic consequence of protein misfolding and aggregation as seen in age-related neurodegenerative diseases. Sirtuin 1 (SIRT1), an NAD(+) -dependent lysine deacetylase with important biological
Mammalian SIRT1 Represses Forkhead Transcription Factors
Motta MC, et al.
Cell, 116, 551-563 (2004)
SIRT 1 Overexpression is Associated with Metastasis of Pancreatic Ductal Adenocarcinoma (PDAC) and Promotes Migration and Growth of PDAC Cells.
Li S
Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 22 (2016)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service