S5512
Streptavidin−Peroxidase from Streptomyces avidinii
lyophilized powder
Synonym(s):
Streptavidin−HRP from Streptomyces avidinii
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0.1 MG
$77.40
0.5 MG
$224.00
1 MG
$380.00
2 MG
$700.00
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Product Name
Streptavidin−Peroxidase from Streptomyces avidinii, lyophilized powder
conjugate
peroxidase conjugate
form
lyophilized powder
composition
Protein, ≥70% E1%/280
technique(s)
direct ELISA: 1:50,000
storage temp.
−20°C
Quality Level
1 of 4
This Item | S2890 | S0677 | S4762 |
|---|---|---|---|
| technique(s) direct ELISA: 1:50,000 | technique(s) - | technique(s) - | technique(s) - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form essentially salt-free, lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| conjugate peroxidase conjugate | conjugate alkaline phosphatase conjugate | conjugate - | conjugate - |
| composition Protein, ≥70% E1%/280 | composition Protein, ≥40% biuret | composition - | composition - |
Analysis Note
The optimal working dilution should be determined empirically using a range of dilutions from a 1 mg/mL stock in buffer.
Application
Biochem/physiol Actions
Streptavidin is an antibiotic that functions by binding to and depleting the essential vitamin biotin from the surrounding environment.[7] Because of its unique properties, streptavidin has found various applications in biological studies, including immunotherapy, immunoassays, hybridization assays, lymphocyte activation, antigen localization, and affinity chromatography.[8]
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Steptavidin is coupled to horseradish peroxidase (HRP) using citrate buffer, pH 6.0, and a modified published procedure to form a 1:1 conjugate. HRP has a molecular mass of ~44kDa and steptavidin has a molecular mass of ~60kDa.[9][10][11]
Streptavidin derives its name from its bacterial source Streptomyces avidinii and from the hen egg-white protein, avidin, which has high affinity to biotin. Its homologous core shares 33% sequence similarity with avidin, as well as sharing a common tetrameric structure.[12] It is a crystalline tetrameric protein, with a molecular weight of 4*15000Da. It binds four molecules of biotin.[7][13] Streptavidin lacks carbohydrate and sulfur-containing amino acids.[8]
Streptavidin is considered as a high-affinity biotin-binding agent, which shows resistance to extreme pH, detergents temperature, denaturants and enzymes,[14] hence it is used in molecular biology and bionanotechnology.
Packaging
Package size based on protein content
Physical form
Lyophilized powder containing citrate buffer salts.
Preparation Note
Labeled with Type VI peroxidase by a modification of the method of O′Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).
Purified by affinity chromatography.
Purified by affinity chromatography.
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