S5816

Sigma-Aldrich

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse

SDF-1α, recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym(s):
SDF-1α/PBSF
MDL number:
Pricing and availability is not currently available.

Quality Level

biological source

mouse

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

form

powder

potency

0.15-0.6 ng/mL ED50

quality

endotoxin tested

mol wt

predicted mol wt ~8 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

impurities

<1 EU/μgtested (LAL test)

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Cxcl12(20315)

General description

Stromal derived factor-1 (SDF-1) is localized to adipocytes.

Application

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse has been used in invasion assay. It has also been used to study the SDF-1 release from chitosan-based nanoparticles.

Biochem/physiol Actions

Stromal derived factor-1 (SDF-1) is an insulin-desensitizing factor, upregulated during both starvation and obesity. Exogenic SDF-1 reduces insulin-mediated signaling and glucose uptake. SDF-1 mediates migration of cortical interneurons and endothelial progenitor cells of the bone marrow through chemotaxis.
SDF-1α/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. SDF-1α and SDF-1β (together also known as SDF-1α/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α..

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

Preparation Note

Recombinant Mouse Stromal Cell-Derived Factor 1α (SDF-1α)/Pre-B Cell Growth Stimulating Factor (PBSF) is produced from a DNA sequence encoding the mature mouse SDF-1α protein sequence (amino acid residues 22-89) expressed in Escherichia coli. Recombinant mouse SDF-1α (68 amino acids) has a predicted molecular mass of approximately 8 kDa.

Analysis Note

The biological activity is measured by its ability to chemoattract cultured human lymphocytes and mouse BaF3 cells transfected with human CXCR4.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

The Cytokine CXCL12 Promotes Basket Interneuron Inhibitory Synapses in the Medial Prefrontal Cortex.
Wu PR, et al.
Cerebral Cortex, 27(9), 4303-4313 (2017)
CXCR4 receptor positive spheroid forming cells are responsible for tumor invasion in vitro
Krohn A, et al.
Cancer Letters, 280, 65-71 (2009)
SDF-1α-induced dual pairs of E-selectin/ligand mediate endothelial progenitor cell homing to critical ischemia
Liu ZJ, et al.
Scientific Reports, 6: 34416 (2016)
SDF-1 Is an Autocrine Insulin-Desensitizing Factor in Adipocytes.
Shin J, et al.
Diabetes, 67(6), 1068-1078 (2018)
Mobilization of mesenchymal stem cells by stromal cell-derived factor-1 released from chitosan/tripolyphosphate/fucoidan nanoparticles
Huang YC, et al.
Acta Biomaterialia, 8, 1048-1056 (2012)

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