S6402
Streptavidin−Cy3™ from Streptomyces avidinii
buffered aqueous solution
Synonym(s):
Streptavidin Cy3
Skip To
conjugate
CY3 conjugate
Quality Level
form
buffered aqueous solution
extent of labeling
3-9 mol fluorochrome per mol protein
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
shipped in
wet ice
storage temp.
2-8°C
1 of 4
This Item | S4762 | S9059 | S9415 |
|---|---|---|---|
| technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 | technique(s) - | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200, 400 |
| form buffered aqueous solution | form essentially salt-free, lyophilized powder | form buffered aqueous glycerol solution | form - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| conjugate CY3 conjugate | conjugate - | conjugate gold conjugate | conjugate - |
General description
Streptavidin is a homotetramer protein. One molecule of streptavidin binds four molecules of biotin by a non-covalent interaction that is essentially irreversible. Streptavidin has a high affinity for biotin and is extensively used as a labeling tool and for studies involving binding proteins.
Application
Streptavidin−Cy3™ from Streptomyces avidinii has been used:
- in double immunofluorescence staining to detect biotinylated lectin
- for insulin and C-peptide staining in immunofluorescence analysis
- in double immunofluorescence to verify the presence of cells filled with biocytin
- in conjunction with biotinylated antibodies in immunohistochemistry and immunocytochemistry
- with the traditional rhodamine filter arrangements due to the spectral overlap
Features and Benefits
Spectral Characteristics of Cy3 Dye:
- Absorbance Max 552 nm
- Emission Max 565 nm
- Molar Extinction Coefficient 1.3 x 10-5 M-1cm-1
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide
Legal Information
Cy3 is a trademark of Cytiva
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Ji-Myung Bae et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 33(6), 1126-1140 (2018-02-07)
The Sp7/Osterix transcription factor is essential for bone development. Mutations of the Sp7 gene in humans are associated with craniofacial anomalies and osteogenesis imperfecta. However, the role of Sp7 in embryonic tooth development remains unknown. Here we identified the functional
Agnieszka E Gorska et al.
The American journal of pathology, 163(4), 1539-1549 (2003-09-26)
We have previously shown that expression of a dominant-negative type II transforming growth factor-beta receptor (DNIIR) in mammary epithelium under control of the MMTV promoter/enhancer causes alveolar hyperplasia and differentiation in virgin mice. Here we show that MMTV-DNIIR female mice
Niklas Schwarz et al.
eLife, 8 (2019-09-10)
Most of our knowledge on human CNS circuitry and related disorders originates from model organisms. How well such data translate to the human CNS remains largely to be determined. Human brain slice cultures derived from neurosurgical resections may offer novel
Hiroyuki Nobusue et al.
Cell and tissue research, 332(3), 435-446 (2008-04-04)
We established a preadipocyte cell line from mature adipocytes obtained from subcutaneous fat tissue of green fluorescent protein (GFP) transgenic mice. The floating top layer, containing mature adipocytes, was isolated from subcutaneous fat tissue by collagenase digestion and filtration. Fluorescence-activated
Miroslav Hons et al.
Nature immunology, 19(6), 606-616 (2018-05-20)
Although much is known about the physiological framework of T cell motility, and numerous rate-limiting molecules have been identified through loss-of-function approaches, an integrated functional concept of T cell motility is lacking. Here, we used in vivo precision morphometry together
Articles
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
Related Content
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service