S7073

SGK1 (60-end), active, GST tagged human

Name: SGK1 (60-end), active, GST tagged human
Brand: SIGMA
Price: 489 USD

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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10 μG

$489.00

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About This Item

UNSPSC Code:

12352200

NACRES:

NA.32

Key Documents

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recombinant

expressed in baculovirus infected Sf9 cells

Quality Level

200

product line

PRECISIO^® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

99-135 nmol/min·mg

mol wt

~73 kDa

UniProt accession no.

O00141

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... SGK1(6446)

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This Item	S8939	S6948	C0499
Sigma-Aldrich S7073 SGK1 (60-end), active, GST tagged human	Sigma-Aldrich S8939 SGK1 (δ1-60, S422D), active human	Sigma-Aldrich S6948 SGK2, active, GST tagged human	Sigma-Aldrich C0499 c-KIT (544-end, V654A), active, GST tagged human
specific activity 99-135 nmol/min·mg	specific activity -	specific activity 70-94 nmol/min·mg	specific activity 6-8 nmol/min·mg
Gene Information human ... SGK1(6446)	Gene Information human ... SGK1(6446)	Gene Information human ... SGK2(10110)	Gene Information human ... KIT(3815)
assay ≥70% (SDS-PAGE)	assay -	assay ≥70% (SDS-PAGE)	assay ≥70% (SDS-PAGE)
form buffered aqueous glycerol solution	form buffered aqueous solution	form buffered aqueous glycerol solution	form buffered aqueous glycerol solution
recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells (truncated human sequence (a.a. 1-60 deleted, S422D mutation)-histidine-tagged)	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells
storage temp. −70°C	storage temp. −70°C	storage temp. −70°C	storage temp. −70°C

Biochem/physiol Actions

SGK1 is a member of the serum- and glucocorticoid-induced protein kinase that is activated in vitro by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and in vivo in response to signals that activate phosphatidylinositol (PI) 3-kinase. SGK1 mRNA is expressed in all tissues and the level of SGK1 mRNA is increased by stimulation with serum or dexamethasone. SGK1 promotes cell survival by phosphorylating and inactivating FKHRL1. SGK and Akt display differences with respect to the efficacy with which they phosphorylate the three regulatory sites on FKHRL1.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

SLBW4715

SLBV5167

SLBS1841V

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Protein kinase SGK mediates survival signals by phosphorylating the forkhead transcription factor FKHRL1 (FOXO3a).

A Brunet et al.

Molecular and cellular biology, 21(3), 952-965 (2001-01-12)

Serum- and glucocorticoid-inducible kinases (SGKs) form a novel family of serine/threonine kinases that are activated in response to a variety of extracellular stimuli. SGKs are related to Akt (also called PKB), a serine/threonine kinase that plays a crucial role in

Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase.

T Kobayashi et al.

The Biochemical journal, 344 Pt 1, 189-197 (1999-11-05)

The catalytic domain of serum- and glucocorticoid-induced protein kinase (SGK) is 54% identical with protein kinase B (PKB) and, like PKB, is activated in vitro by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and in vivo in response to signals that activate phosphatidylinositol

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